REGULATION OF LYMPHOCYTE MIGRATION BY MACROPHAGES

巨噬细胞对淋巴细胞迁移的调节

• 批准号: 3445490
• 负责人: HOU T CHEUNG
• 金额: $ 4.34万
• 依托单位: ILLINOIS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1987-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3445490
• 关键词: T lymphocyte; cell cell interaction; cell migration; cell population study; chemoattractants; immunochemistry; immunoregulation; leukocyte activation /transformation; macrophage; tissue /cell culture

The regulation of lymphocyte migration by macrophages will be studied in vitro using the random migration assay and the modified Boyden chemotaxis assay. The culture supernatants of peritoneal macrophages contain a factor(s) that is(are) chemokinetic and chemotactic for lymphocytes as observed in our laboratory. The chemokinetic activity is also present in the culture supernatants of J744A and P388D1 macrophage-like cell lines. In the proposed project the macrophage factor(s)1 will be isolated, purified and characterized, and its effect on the migration of different lymphocyte populations will be studied using lymphocyte subpopulations isolated by conventional methods and using cloned T cells. The in vivo effect of the purified factor(s) on the migration and distribution of lymphocytes will also be studied. The mechanisms of action of the factor(s) on the modulation of lymphocyte migration will be examined by studying the changes in the contractile and cytoskeletal structure of the affected cells. The proposed study is intended to gain new information on the mechanism and regulation of lymphocyte migration and localization.

将研究通过巨噬细胞迁移的淋巴细胞迁移的调节 体外使用随机迁移测定和修饰的Boyden趋化性 测定。 腹膜巨噬细胞的培养上清液包含 因子（s）是（）趋化因子和趋化因子的淋巴细胞趋化因子 在我们的实验室中观察到。 趋化活性也存在于 J744A和P388D1巨噬细胞样细胞系的培养上清液。 在拟议的项目中，将分离巨噬细胞因子1 纯化和特征，及其对不同的迁移的影响 将使用淋巴细胞亚群来研究淋巴细胞种群 通过常规方法分离并使用克隆的T细胞分离。 体内 纯化因子对迁移和分布的影响 还将研究淋巴细胞。 行动机制 淋巴细胞迁移调节的因子将通过 研究收缩和细胞骨架结构的变化 受影响的细胞。 拟议的研究旨在获取有关的新信息 淋巴细胞迁移和定位的机制和调节。

项目成果

Adhesion of lymphoid cells to the carboxyl-terminal heparin-binding domains of fibronectin.

淋巴细胞与纤连蛋白羧基末端肝素结合域的粘附。

• DOI: 10.1016/0014-4827(89)90093-1
• 发表时间: 1989
• 期刊: Experimental cell research
• 影响因子: 3.7
• 作者: Liao,NS;STJohn,J;McCarthy,JB;Furcht,LT;Cheung,HT
• 通讯作者: Cheung,HT

Haptotactic activity of fibronectin on lymphocyte migration in vitro.

纤连蛋白对淋巴细胞体外迁移的趋触活性。

• DOI: 10.1016/0008-8749(90)90187-v
• 发表时间: 1990
• 期刊: Cellular immunology
• 影响因子: 4.3
• 作者: Davis,JM;StJohn,J;Cheung,HT
• 通讯作者: Cheung,HT

Adhesion of lymphoid cell lines to fibronectin-coated substratum: biochemical and physiological characterization and the identification of a 140-kDa fibronectin receptor.

淋巴细胞系对纤连蛋白包被的基质的粘附：生化和生理学特征以及 140-kDa 纤连蛋白受体的鉴定。

• DOI: 10.1016/0014-4827(87)90164-9
• 发表时间: 1987
• 期刊: Experimental cell research
• 影响因子: 3.7
• 作者: Liao,NS;StJohn,J;Du,ZJ;Cheung,HT
• 通讯作者: Cheung,HT

Regulation of lymphocyte motility by macrophages: characterization of a lymphocyte migration inhibitory factor derived from a macrophage-like cell line.

巨噬细胞调节淋巴细胞运动：源自巨噬细胞样细胞系的淋巴细胞迁移抑制因子的表征。

• DOI: 10.1016/0008-8749(89)90163-9
• 发表时间: 1989
• 期刊: Cellular immunology
• 影响因子: 4.3
• 作者: Ye,S;Cheung,HT
• 通讯作者: Cheung,HT

Modulation of lymphocyte motility by macrophages.

巨噬细胞调节淋巴细胞运动。

• DOI: 10.1016/0008-8749(87)90313-3
• 发表时间: 1987
• 期刊: Cellular immunology
• 影响因子: 4.3
• 作者: Cheung,HT;Twu,JS
• 通讯作者: Twu,JS