GROWTH HORMONE AND IMMUNOLOGICAL COMPETENCE

生长激素和免疫能力

• 批准号: 2018087
• 负责人: HOU T CHEUNG
• 金额: $ 9.19万
• 依托单位: ILLINOIS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-26 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2018087
• 关键词: CD34 molecule; CD4 molecule; CD8 molecule; T lymphocyte; apoptosis; bone marrow; cell differentiation; cell population study; flow cytometry; gene expression; hormone receptor; immunosenescence; laboratory rat; protooncogene; somatotropin; thymus

DESCRIPTION (Adapted from the applicant's abstract): The proposal consists of three objectives. First, Is GH required for maintenance of thymic and bone marrow cellularity? Does GH ablation result in reduced cellularity of thymus and bone marrow? The applicant predicts that in the absence of GH there will be reduction in thymic size involving loss of cortical thymocytes and accumulation of CD4- CD8- T cell progenitors. The reduction in bone marrow cellularity will be accompanied by a reduction in multipotent myeloid progenitors. He predicts that these changes will be reversed by GH administration and that this will establish that GH ablation is a valid model for immunosenescence. Second, does GH act as a survival factor in lymphoid and myeloid progenitors in vivo by preventing apoptosis? The applicant hypothesizes that GH promotes survival by maintaining expression of the protooncogene bcl-2 and that withdrawal of GH will result in induction of apoptosis in thymocytes and myeloid progenitors associated with a decrease in BCL-2 levels. Third, the applicant will use highly purified CD34+ cells and flow cytometry to ask if pluripotent hematopoietic progenitors express receptors for GH and IGF-I. Collectively it is hypothesized that these experiments will establish the concept that GH maintains lymphoid and myeloid tissues in vivo by promoting the survival of lymphoid and myeloid progenitors and thus will provide new and important insights into aging of the immune system.

描述（根据申请人的摘要改编）：该提案包括 三个目标。 首先，维持胸腺和 骨髓细胞？ GH消融会导致细胞降低 胸腺和骨髓？ 申请人预测，在没有GH的情况下 胸腺大小将减少涉及皮质胸腺细胞的损失 和CD4-CD8-T细胞祖细胞的积累。 骨减少 骨髓细胞将伴随着多能髓样的降低 祖先。 他预测这些变化将被GH逆转 管理，这将确定GH消融是有效的 免疫衰老的模型。 其次，GH充当淋巴样和髓样祖细胞中的生存因子 通过预防凋亡来体内？ 申请人假设GH 通过维持原子能元Bcl-2的表达来促进生存 GH的戒断将导致胸腺细胞凋亡 和与Bcl-2水平降低有关的髓样祖细胞。 第三，申请人将使用高度纯化的CD34+细胞和流式细胞仪 询问多能造血祖细胞是否表达GH和GH的受体 IGF-i。 总的来说，这些实验将建立 GH通过促进在体内维持淋巴样和髓样组织的概念 淋巴机和髓样祖细胞的生存，因此将提供新的 以及对免疫系统衰老的重要见解。