NUTRITION, AGING, AND IMMUNITY

营养、衰老和免疫力

• 批准号: 3068433
• 负责人: HOU T CHEUNG
• 金额: $ 7.18万
• 依托单位: ILLINOIS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1992-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3068433
• 关键词: T lymphocyte; autoradiography; caloric dietary content; cell age; clone cells; complementary DNA; gene expression; histochemistry /cytochemistry; immunochemistry; interleukin 2; interleukin 3; laboratory rat; leukocyte activation /transformation; longevity; messenger RNA; molecular genetics; nucleic acid probes; nutrition related tag; tissue /cell culture

The classic study by McCay demonstrates that dietary restriction can prolong life-span and reduce incidence of age-specific diseases. This and other similar studies suggest that nutritional manipulation could be applied to the human populations not only to increase life-expectancy, but also to avoid debilitating disease associated with old age. However, before such an application is possible, it is important to understand the theoretical principles underlying this process. I am interested in the effect of dietary restriction on the immune system during aging because of the role of the immune system in the defense against infectious diseases, malignancies, and autoimmunity. Furthermore, the decline of the immune system is demonstrated to be responsible for the increased susceptibility to these diseases. Dietary restriction not only reduces disease incidence, but it can also restore many immune functions. However, the mechanism responsible for this process is unclear. I have previously shown that lymphocyte functions decline with age as determined by their reduced proliferative capacity. Subsequent biochemical and genetic studies, found that the decline in interleukin 2 and interleukin 3 synthesis and genetic expression by helper T cells might be responsible. In this proposal, I will use the same molecular approach to elucidate the relationship between the physiological and molecular processes in T cell activation under the influence of dietary restriction. In addition, I will take a new approach in addressing the question on cellular aging by establishing cloned T cell lines from rats of different ages either with or without dietary restriction. My training in immunology, my research experience in gerontology, and my interest in dietary restriction provide me the suitable background to apply for the SERCA award.

McCay的经典研究表明饮食限制 可以延长寿命并降低特定年龄的发生率 疾病。 这项和其他类似研究表明营养 操纵不仅可以应用于人口 增加人生的预期，但也避免使疾病使人衰弱 与老年有关。 但是，在这样的申请是 可能，重要的是要了解理论原理 这一过程的基础。 我对饮食的影响感兴趣 衰老期间对免疫系统的限制 防御感染疾病的免疫系统， 恶性肿瘤和自身免疫性。 此外，衰落 事实证明免疫系统负责 增加对这些疾病的敏感性。 饮食限制不是 只会降低疾病的发病率，但也可以恢复许多 免疫功能。 但是，为此负责的机制 过程尚不清楚。 我以前已经表明淋巴细胞 功能随着年龄的增长而下降 增殖能力。 随后的生化和遗传 研究发现，白介素2和白介素3的下降3 辅助T细胞的合成和遗传表达可能是 负责任的。 在此提案中，我将使用相同的分子 阐明生理之间关系的方法 在影响下T细胞激活中的分子过程 饮食限制。 此外，我将采取一种新的方法 通过建立克隆T来解决有关细胞衰老的问题 来自不同年龄的大鼠的细胞系有或没有 饮食限制。 我的免疫学培训，我的研究 老年病的经验以及我对饮食限制的兴趣 为我提供适合申请SERCA的背景 奖。