MRI OF FETAL NEURAL GRAFTS IN INJURED SPINAL CORDS

受损脊髓中胎儿神经移植物的 MRI

• 批准号: 3416149
• 负责人: THOMAS H. MARECI
• 金额: $ 6.59万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-05-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3416149
• 关键词: cats; disease /disorder model; histocompatibility; histology; lipids; nervous system disorder diagnosis; nervous system regeneration; nervous system transplantation; neuroanatomy; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; spinal cord injury

DESCRIPTION: (Adapted from the application) The goal of the research proposed is to assess the extent of spinal cord damage, and evaluate possible repair following transplantation of fetal spinal cord tissue, by using high-field MR imaging and spectral imaging in vivo. This study will assess on a macroscopic level the survival and development of fetal transplants and their interactions with the host spinal cord in a living animal. The following specific aims will be addressed. Aim 1 is to improve on images currently being obtained with conventional MR imaging methods. The applicants will develop high spatial-resolution images of the injured spinal cord to provide more detailed observations of post-traumatic pathology in vivo. In addition, they will explore whether three-dimensional image reconstructions of spinal lesions can be generated from serial and volume MR slices. Aim 2 will test the ability of H-1 MR spectral imaging to determine the tissue distribution of water and lipid. The focus of this aim will be to relate anatomical and gross chemical changes taking place in both injured cord and transplant tissue. Aim 3 is to study the dynamics of transplant growth and maturation in the acutely- and chronically-injured spinal cord using the methods developed in Aim 1 and Aim 2. Current analyses of fetal CNS transplants and their interactions with host spinal cord have been largely limited to post-mortem histological studies. Examination of spinal cord injury and repair with MR imaging and spectral (MRS) imaging may provide a tool for determining in vivo whether a transplant procedure will be successful.

描述：（从应用程序改编）研究的目标 提出的是评估脊髓损伤的程度，并评估 通过移植胎儿脊髓组织后可能的维修 在体内使用高场MR成像和光谱成像。 这项研究会 宏观评估胎儿的生存和发展 移植物及其与宿主脊髓的相互作用 动物。 将解决以下特定目标。 目标1是 改进使用常规MR成像获得的目前获得的图像 方法。 申请人将开发高空间分辨率图像 受伤的脊髓可提供创伤后的更详细的观察 体内病理学。 此外，他们将探索是否 可以生成脊柱病变的三维图像重建 从串行和卷MR SLICS。 AIM 2将测试H-1 MR的能力 光谱成像以确定水和脂质的组织分布。 该目标的重点是关联解剖学和总化学 受伤的绳索和移植组织都发生了变化。 AIM 3是 研究移植生长和成熟的动力学 并使用AIM 1中开发的方法长期损坏了脊髓 目标2。胎儿中枢神经系统移植及其的当前分析 与宿主脊髓的相互作用在很大程度上仅限于验尸 组织学研究。 检查脊髓损伤和MR修复 成像和光谱（MRS）成像可能会提供确定的工具 Vivo是否会成功进行移植程序。