MR EVALUATION OF SPINAL CORD INJURY AND REPAIR

脊髓损伤和修复的 MR 评估

基本信息

批准号：
6094794
负责人：
THOMAS H. MARECI
金额：
$ 2.5万
依托单位：
UNIVERSITY OF FLORIDA
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
起止时间：
1991-05-01 至 2000-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6094794
关键词：
biomedical equipment development cats cell transplantation diagnosis design /evaluation embryo /fetus cell /tissue histopathology immunocytochemistry laboratory rat neurochemistry nuclear magnetic resonance spectroscopy prognosis spinal cord imaging /visualization spinal cord injury spinal cord surgery

项目摘要

This proposal addresses the development of nuclear magnetic resonance (NMR) spectroscopy and imaging techniques for application to in vivo studies of spinal cord injury (SCI) and functional repair. Three aims are proposed that will test the hypothesis that serial, non-invasive NMR measurements in individual animals can identify tissue parameters that reflect the time-course of lesion stabilization as well as the success of intraspinal grafting methods. The first aim is to develop: (a) diffusion- sensitive NMR imaging; (b) localized 1/H NMR spectroscopy; and (c) implanted radiofrequency (RF) reception coils that can be used to monitor tissue dynamics associated with spinal cord injury and transplantation. These NMR methods will then be used to address specific biological issues pertaining to spinal cord injury and repair with intraspinal fetal grafts. Therefore, the second aim is to obtain reliable, repeated measures of several anatomical (diffusion weighted images, T/1 and T/2 mapping) and neurochemical indices (1/H NMR spectra of lipids, lactate, N-acetyl compounds, and choline) of SCI. This information will be used to: (a) establish the evolution of the lesion; (b) determine the time after injury at which stabilization of spinal cord pathophysiology occurs; and (c) investigate the extent to which various NMR indices of lesion development and biochemical change correlate with functional outcome. The last aim of this project involves studies to: (a) evaluate the maturation, host-graft integration and long-term survival of intraspinal grafts of fetal neural tissue using the NMR approaches outlined in Aim 2; and (b) correlate these NMR parameters with histological assessments of host and graft tissues and measures of locomotor performance.

该提案解决了核磁共振的发展（NMR）用于在体内应用的光谱和成像技术脊髓损伤（SCI）和功能修复的研究。三个目标是提出的将检验串行，无创NMR的假设单个动物的测量可以鉴定组织参数反映病变稳定的时间顺序以及成功载体内移植方法。第一个目的是开发：（a）扩散 - 敏感的NMR成像；（b）局部1/h NMR光谱；（c）植入的射频（RF）接收线圈，可用于监测与脊髓损伤和移植有关的组织动力学。然后，这些NMR方法将用于解决特定的生物学问题与脊髓损伤有关，并用脊髓内胎儿移植物进行修复。因此，第二个目的是获得可靠的，重复的措施几个解剖学（扩散加权图像，T/1和T/2映射）和神经化学指数（脂质的1/H NMR光谱，乳酸，N-乙酰基科学的化合物和胆碱。此信息将用于：（a）建立病变的演变；（b）确定受伤后的时间发生脊柱病理生理学的稳定；（c）研究病变发展的各种NMR指数的程度生化变化与功能结果相关。最后的目标该项目涉及研究：（a）评估成熟的宿主移植胎儿神经内脊椎内移植物的整合和长期存活使用AIM 2中概述的NMR接近组织；（b）将这些关联 NMR参数具有对宿主和移植组织的组织学评估以及运动性能的度量。