IMMUNOCYTOCHEMICAL ORGANIZATION OF MEDIAN EMINENCE

中位突出的免疫细胞化学组织

• 批准号: 3399811
• 负责人: ROBERT Y. MOORE
• 金额: $ 8.96万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-07-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3399811
• 关键词: axon; electron microscopy; histochemistry /cytochemistry; immunochemistry; median eminence; neuroanatomy; neurochemistry; neuroendocrine system; neurotransmitters

The principal objective of this research program is to analyze the ultrastructural organization of chemically identified systems of axons in the median eminence of the rat. The proposal consists of three major sections. In the first portion of the proposal, the normal ultrastructural organization of the median eminence will be studied with fine structrual morphometric techniques. Four levels throughout the rostrocaudal axis of the median eminence have been chosen for the analysis on the basis of previous investigations which have demonstrated differential distribution of chemically distinct systems of axons in the median eminence. In the second portion of the program, electron microscopic immunoperoxidase techniques will be utilized to study the organization of individual systems of axons in the median eminence. Antibodies generated against the following antigens are available for these studies: vasopressin, oxytocin, somatostatin, avian pancreatic polypeptide, tyrosine hydroxylase, glutamic acid decarboxylase, luteinizing hormone releasing hormone, serotonin, B-endorphin, adrenocorticotropin releasing hormone, Alpha-melanocyte stimulating hormone, leu-enkephalin and neurotensin. In the final phase of the investigation, attempts will be made to perfect techniques which will allow localization of two or more antigens within a single ultrathin section in order that the interrelation of dense and overlapping, although chemically distinct, systems of axons can be studied.

该研究计划的主要目的是分析 化学确定轴突系统的超微结构组织 大鼠的中位数。 该提案由三个主要 部分。 在提案的第一部分中，正常的超微结构 中位数的组织将通过细分研究进行研究 形态计量技术。 在整个主尾轴的四个级别 基于 以前证明分布差分的调查 轴突中位数的化学不同系统。 在 该程序的第二部分，电子显微镜免疫过氧化物酶 技术将用于研究单个系统的组织 轴突中位数。 产生的抗体 以下抗原可用于这些研究：加压素，催产素， 生长抑素，鸟类胰腺多肽，酪氨酸羟化酶，谷氨酸 酸脱羧酶，黄体生成激素释放激素，5-羟色胺， B-内啡肽，肾上腺皮质激素释放激素，α-甲状腺细胞 刺激激素，Leu-Enkephalin和Neurotensin。 在最后阶段 调查，将尝试进行完美的技术 允许将两个或多个抗原定位在单个超薄中 部分为了使密集和重叠的相互关系，尽管 化学上不同的轴突系统可以研究。