HORMONAL INFLUENCES ON THE NEUROBIOL OF EXCITABLE CELLS

激素对兴奋细胞神经生物学的影响

• 批准号: 3400067
• 负责人: ANDREW H BASS
• 金额: $ 7.25万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1987-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3400067
• 关键词: autoradiography; central nervous system; dihydrotestosterone; electrolytes; electron microscopy; electrophysiology; estradiol; gender difference; hormone regulation /control mechanism; membrane structure; molecular site; neural information processing; neuroanatomy; neurochemistry; neuroendocrine system; saltwater environment; steroid hormone; testosterone

We are studying the influences of gonadal hormones on the anatomy and physiology of the excitable cells of the electric organ, electroreceptors, and the central electromotor pathway of the weakly electric fishes, Mormyridae. The electric organ produces a species-typical electrical signal, the Electric Organ Discharge (E O D), which is important for mechanisms of species and sex recognition. The characteristic properties of the EOD waveform depend upon the anatomy and physiology of the electrocytes, the excitable cells of the electric organ. We have discovered that gonadal steroid hormones can induce a male-type EOD waveform among juveniles and females of species with a natural sex difference in their EOD waveform. A special set of electroreceptors, that produce spike-like receptor potentials, show a difference in frequency tuning between natural females and, natural males or females with a hormonally-induced male EOD waveform. We will study the mechanisms underlying hormone-induced changes in EOD waveforms and electroreceptor tuning with electrophysiological, anatomical, and biochemical techniques. We have also identified, using steroid autoradiography, hormone-concentrating cells in the central electromotor pathway. These findings will be extended with additional autoradiographic studies. Since the central pathway controls the rate of electric organ excitation, i.e. the EOD rhythm, we will examine possible differences in the EOD rhythm between natural males and females, and hormone-treated specimens.

我们正在研究性腺激素对解剖学和 电器官，电受体的激发细胞的生理学 以及弱电鱼的中央电动途径， 摩米丽科。 电器官产生物种典型的电信号， 电器器排放（E O D），这对于机制很重要 物种和性别识别。 EOD的特性 波形取决于电细胞的解剖学和生理学， 电孔的可激发细胞。 我们发现性腺 类固醇激素可以在少年中诱导雄性型EOD波形和 具有自然性别差异的物种女性的EOD波形。 一个 产生尖峰样受体的特殊电动受体集 电势，显示自然女性之间的频率调整差异 以及具有荷尔蒙引起的雄性EOD波形的天然雄性或女性。 我们将研究激素诱导的EOD变化的基础机制 带有电生理，解剖学的波形和电受体调节 和生化技术。 我们还使用类固醇放射自显影，确定 中央电气途径中的激素浓缩细胞。 这些 研究结果将通过其他放射自显影研究扩展。 自从 中央途径控制电器官激发的速率，即 EOD节奏，我们将检查EOD节奏的可能差异 在天然雄性和女性之间，以及激素处理的标本。