Beating Hearts at High Resolution: Adaptive High Resolution Selective Plane Illumination Microscopy

高分辨率下的心跳：自适应高分辨率选择性平面照明显微镜

• 批准号: EP/I010173/1
• 负责人: Gordon Love
• 金额: $ 64.54万
• 依托单位: Durham University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2011
• 资助国家: 英国
• 起止时间: 2011 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FI010173%2F1
• 关键词: Beating; Hearts; Resolution; Adaptive; Selective

In this project we will film life as it develops in the heart by exploiting developments in optical microscopy, high speed imaging, and adaptive optics. The hearts will be in live, developing zebrafish and mouse embryos. This is extremely challenging as the living heart is large (on a microscopic scale), is embedded in the body of the animal (meaning it must be imaged at depth) and is beating, and therefore moving, at a rate of around 300 beats per minute. As well as providing a challenge to demonstrate the advanced methods we will develop the study of cardiac development is important both for investigating the development of the healthy heart and for determining the causes of heart problems.

在这个项目中，我们将通过利用光学显微镜，高速成像和自适应光学元件中的发展来拍摄生活中的生命。心脏将在现场发展，发展斑马鱼和小鼠胚胎。这是极具挑战性的，因为活着的心脏很大（在微观尺度上），嵌入了动物的体内（这意味着它必须在深度成像）中，并且以每分钟约300次的速度跳动，因此移动。除了提供挑战以证明先进的方法，我们将开发心脏发展研究对于研究健康心脏的发展以及确定心脏问题的原因而言，这既重要。

项目成果

In Smooth Muscle, Mitochondrial Movement is Restricted in Native Cells and Unrestricted and Trafficked When Cells are in Culture

在平滑肌中，线粒体运动在天然细胞中受到限制，而当细胞处于培养状态时则不受限制和运输

• DOI: 10.1016/j.bpj.2009.12.1617
• 发表时间: 2010
• 期刊: Biophysical Journal
• 影响因子: 3.4
• 作者: Chalmers S

Precise spatio-temporal control of rapid optogenetic cell ablation with mem-KillerRed in Zebrafish.

• DOI: 10.1038/s41598-017-05028-2
• 发表时间: 2017-07-11
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Buckley C;Carvalho MT;Young LK;Rider SA;McFadden C;Berlage C;Verdon RF;Taylor JM;Girkin JM;Mullins JJ
• 通讯作者: Mullins JJ

3D adaptive optics in a light sheet microscope

• DOI: 10.1364/oe.20.013252
• 发表时间: 2012-06-04
• 期刊: OPTICS EXPRESS
• 影响因子: 3.8
• 作者: Bourgenot, Cyril;Saunter, Christopher D.;Love, Gordon D.
• 通讯作者: Love, Gordon D.

Adaptive optics for wide-field microscopy

用于宽视场显微镜的自适应光学器件

• DOI: 10.1117/12.873857
• 发表时间: 2011
• 作者: Bourgenot C

Light sheet adaptive optics microscope for 3D live imaging

• DOI: 10.1117/12.2002057
• 发表时间: 2013-01-01
• 期刊: THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XX
• 作者: Bourgenot, C.;Taylor, J. M.;Love, G. D.
• 通讯作者: Love, G. D.