FETAL LUNG BIOCHEMISTRY AND 3-DIMENSIONAL MICROANATOMY

胎肺生物化学和三维显微解剖学

• 批准号: 3343492
• 负责人: Stephen L. Young
• 金额: $ 16.11万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-05-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3343492
• 关键词: Golgi apparatus; artificial intelligence; biochemistry; bronchus; cell cell interaction; cell differentiation; cell morphology; collagen; computer graphics /printing; density gradient ultracentrifugation; elastin; electron microscopy; embryo /fetus; embryo /fetus drug adverse effect; gestational age; glucocorticoids; laboratory rat; longitudinal animal study; lung; lung alveolus; morphology; phospholipids; photography; postnatal growth disorder; pulmonary surfactants; respiratory function; secretion; stainings; thin layer chromatography

During late fetal development, the lung must prepare for the transition to air breathing. Maintaining alveolar stability becomes vital for survival. Therapy with glucocorticoids to enhance lung maturity may have immediate and long term consequences for lung structure and function. This series of investigations is directed toward furthering our basic knowledge of lung secretory cell development. We will use structural and biochemical methods to test hypotheses of secretory cell function and to test the probabilities that glucocorticoids damage fetal and newborn lung structure. We intend to study biochemical and 3-dimensional morphologic changes during epithelial cell maturation in the last days of fetal development. We will test hypotheses of the assembly of lamellar bodies and assess the changes in type II cell maturation induced by glucocorticoids. This drug induced accelerated maturation is a relevant clinical concern as the effects are widespread within the lung and have not been shown to be entirely reversible during postnatal growth. A detailed 3-dimensional morphologic study of groups of alveolar septa and of terminal bronchiolar structure will be conducted at allow magnification level that will test the microanatomic basis for theories of cell-cell interactions.

在胎儿发育后期，肺必须为过渡做好准备 空气呼吸。 保持肺泡稳定性对于生存至关重要。 使用糖皮质激素治疗以增强肺成熟度可能会立即产生效果 以及对肺结构和功能的长期影响。 这个系列的 研究旨在进一步加深我们对肺的基础知识 分泌细胞发育。 我们将使用结构和生化方法 测试分泌细胞功能的假设并测试概率 糖皮质激素会损害胎儿和新生儿的肺结构。 我们打算 研究上皮细胞生长过程中的生化和三维形态变化 胎儿发育最后几天的细胞成熟。 我们将测试 层状体组装的假设并评估 糖皮质激素诱导II型细胞成熟。 这种药物诱发 加速成熟是一个相关的临床问题，因为其影响 广泛存在于肺部，但尚未被证明完全 产后生长过程中可逆。 详细的 3 维形态学 肺泡间隔组和终末细支气管结构的研究 将在允许的放大水平下进行，这将测试 细胞与细胞相互作用理论的微观解剖学基础。