FETAL LUNG BIOCHEMISTRY AND MICROANATOMY

胎肺生物化学和显微解剖学

• 批准号: 6388935
• 负责人: Stephen L. Young
• 金额: $ 8.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-05-01 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6388935
• 关键词: antisense nucleic acid; cytokine receptors; developmental genetics; embryo /fetus cell /tissue; extracellular matrix; fibroblasts; immunocytochemistry; in situ hybridization; laboratory rat; lung; lung alveolus; lung injury; mixed tissue /cell culture; monoclonal antibody; nonmammalian vertebrate embryology; nucleic acid probes; perinatal; polymerase chain reaction; protein biosynthesis; receptor expression; respiratory epithelium; transforming growth factors

DESCRIPTION (Adapted from the applicant's abstract): The investigators hypothesize that the cellular responses to local and distant production of TGF-b are set by the expression of specific receptors for the cytokine. Aim 1 is to determine the temporal and spatial expression of rat lung TGFR during ontogeny. Aim 2 is to correlate the expression of rat lung TGFR during development with their expression during the repair of perinatal lung injury. Aim 3 is to test whether TGFRI or TGFRII controls production of ECM components by expressing mutated TGFRI and TGFRII in fibroblasts cultured from lungs of fetal rats. Aim 4 is to test the role of fibroblast TGFR expression as instruction for lung development.

描述（改编自申请人的摘要）：研究人员 假设细胞对局部和远处产生的反应 TGF-b 由细胞因子特异性受体的表达决定。 目的 图1是测定大鼠肺TGFR的时空表达 个体发育期间。 目标 2 是关联大鼠肺 TGFR 的表达 发育过程中及其在围产期肺修复过程中的表达 受伤。 目标 3 是测试 TGFRI 或 TGFRII 是否控制 ECM 的产生 通过在培养的成纤维细胞中表达突变的 TGFRI 和 TGFRII 成分 来自胎鼠的肺部。 目的4是测试成纤维细胞TGFR的作用 表达作为肺发育的指示。

项目成果

Requirement of transforming growth factor-beta (TGF-beta) type II receptor for TGF-beta-induced proliferation and growth inhibition.

TGF-β 诱导的增殖和生长抑制需要转化生长因子-β (TGF-β) II 型受体。

• DOI: 10.1074/jbc.271.5.2369
• 发表时间: 1996
• 期刊: The Journal of biological chemistry
• 作者: Zhao,Y;Young,SL
• 通讯作者: Young,SL

Effect of upright posture on the aldosterone responses to dopamine, metoclopramide, angiotensin II, and adrenocorticotropin.

直立姿势对醛固酮对多巴胺、甲氧氯普胺、血管紧张素 II 和促肾上腺皮质激素反应的影响。

• DOI: 10.1210/jcem-65-1-203
• 发表时间: 1987
• 期刊: The Journal of clinical endocrinology and metabolism
• 作者: Malchoff,CD;Hughes,JM;Carey,RM
• 通讯作者: Carey,RM

Expression of transforming growth factor-beta receptors during hyperoxia-induced lung injury and repair.

高氧诱导的肺损伤和修复过程中转化生长因子-β受体的表达。

• DOI: 10.1152/ajplung.1997.273.2.l355
• 发表时间: 1997
• 期刊: The American journal of physiology.
• 作者: Zhao,Y;Gilmore,BJ;Young,SL
• 通讯作者: Young,SL

Ontogeny and localization of TGF-beta type I receptor expression during lung development.

肺发育过程中 TGF-β I 型受体表达的个体发育和定位。

• DOI: 10.1152/ajplung.2000.278.6.l1231
• 发表时间: 2000
• 期刊: American journal of physiology. Lung cellular and molecular physiology
• 作者: Zhao,Y;Young,SL;McIntosh,JC;Steele,MP;Silbajoris,R
• 通讯作者: Silbajoris,R

Surfactant apoprotein in adult rat lung compartments is increased by dexamethasone.

地塞米松可增加成年大鼠肺室中的表面活性剂脱辅基蛋白。

• DOI: 10.1152/ajplung.1991.260.2.l161
• 发表时间: 1991
• 期刊: The American journal of physiology
• 作者: Young,SL;Ho,YS;Silbajoris,RA
• 通讯作者: Silbajoris,RA