FETAL LUNG BIOCHEMISTRY AND 3-DIMENSIONAL MICROANATOMY

胎肺生物化学和三维显微解剖学

• 批准号: 3343486
• 负责人: Stephen L. Young
• 金额: $ 16.06万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-05-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3343486
• 关键词: Golgi apparatus; artificial intelligence; biochemistry; bronchus; cell cell interaction; cell differentiation; cell morphology; computer graphics /printing; density gradient ultracentrifugation; electron microscopy; embryo /fetus; embryo /fetus drug adverse effect; gestational age; glucocorticoids; longitudinal animal study; lung; lung alveolus; morphology; phospholipids; photography; postnatal growth disorder; pulmonary surfactants; respiratory function; secretion; stainings; thin layer chromatography

During late fetal development, the lung must prepare for the transition to air breathing. Maintaining alveolar stability becomes vital for survival. Therapy with glucocorticoids to enhance lung maturity may have immediate and long term consequences for lung structure and function. This series of investigations is directed toward furthering our basic knowledge of lung secretory cell development. We will use structural and biochemical methods to test hypotheses of secretory cell function and to test the probabilities that glucocorticoids damage fetal and newborn lung structure. We intend to study biochemical and 3-dimensional morphologic changes during epithelial cell maturation in the last days of fetal development. We will test hypotheses of the assembly of lamellar bodies and assess the changes in type II cell maturation induced by glucocorticoids. This drug induced accelerated maturation is a relevant clinical concern as the effects are widespread within the lung and have not been shown to be entirely reversible during postnatal growth. A detailed 3-dimensional morphologic study of groups of alveolar septa and of terminal bronchiolar structure will be conducted at allow magnification level that will test the microanatomic basis for theories of cell-cell interactions.

在晚期发育期间，肺必须为过渡做准备 空气呼吸。 维持牙槽稳定对于生存至关重要。 糖皮质激素治疗以增强肺成熟度可能立即具有 肺结构和功能的长期后果。 这个系列 调查旨在促进我们对肺的基本知识 分泌细胞的发展。 我们将使用结构和生化方法 测试分泌细胞功能的假设并测试概率 糖皮质激素会损害胎儿和新生儿肺结构。 我们打算 研究上皮期间的生化和三维形态变化 在胎儿发育的最后几天的细胞成熟。 我们将测试 层状体组装的假设并评估变化 糖皮质激素诱导的II型细胞成熟。 该药物引起的 加速成熟是一个相关的临床问题，因为影响是 肺部广泛，尚未被证明完全是 在产后生长期间可逆。 详细的三维形态学 牙槽隔和末端支气管结构组的研究 将以允许放大级别进行测试 细胞 - 细胞相互作用理论的微解剖基础。