BACTERIAL MINICELL MUTANTS

细菌小细胞突变体

• 批准号: 3300500
• 负责人: LAWRENCE I ROTHFIELD
• 金额: $ 18.49万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-01 至 1992-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3300500
• 关键词: DNA replication; Escherichia coli; cell cycle; crosslink; gene expression; guanine nucleotide binding protein; immunocytochemistry; immunoelectron microscopy; laboratory rabbit; microorganism growth; organelles; temperature sensitive mutant

The long-range objective of this project is to understand the mechanism used by bacterial cells to place the division site at its proper location within the cell. To approach this goal, the bacterial minicell locus (minB) will be studied since mutation of this locus results in aberrant placement of the division septum. The studies are based on recent work showing that the miniB locus codes for several genes that affect the division process. The studies will use a combination of genetics, biochemistry and immunoelectronmicroscopy to localize the minB gene products within the cell, to identify their interactions with each other and with other protein components of the cell division machinery, to probe for possible enzymatic functions of one of the proteins, and to examine the interaction of the minB system with another gene product (dicB) whose effect on the cell division process is likely to be mediated by the minB system.

该项目的长期目标是了解 细菌细胞将分裂位点置于其自身位置的机制 细胞内的正确位置。 为了实现这一目标， 细菌小细胞基因座（minB）将被研究，因为突变 该位点导致分裂隔膜的异常位置。 这些研究基于最近的工作，表明 miniB 基因座 编码影响分裂过程的几个基因。 这 研究将结合遗传学、生物化学和 免疫电子显微镜定位 minB 基因产物 细胞，以确定它们之间的相互作用以及与 细胞分裂机制的其他蛋白质成分，以探测 了解其中一种蛋白质可能的酶功能，并 检查 minB 系统与另一个基因的相互作用 产品（dicB）可能对细胞分裂过程产生影响 由 minB 系统介导。