Studies of E. coli Min proteins

大肠杆菌 Min 蛋白的研究

• 批准号: 8131267
• 负责人: LAWRENCE I ROTHFIELD
• 金额: $ 14.44万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-16 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8131267
• 关键词: Bacteria; Behavior; Cell Cycle; Cell division; Cells; Chromosome Segregation; Elements; Environmental Wind; Escherichia coli; Filament; Fluorescence Microscopy; Genetic; Goals; Grant; Mind; Mining; Mutation; Pattern; Play; Process; Proteins; Role; Site; Specificity; Structure; System; Work; preference; protein structure function

The long-range goal of this project is to determine the mechanism used by bacteria to select the proper cell division site at midcell. The three Min proteins, MinC, MinD and MinE, are required for proper site selection. The proteins have a unique cellular localization pattern and undergo a unique pole-to-pole oscillatory cycle. We have recently shown that the MinCDE proteins are organized into spiral filaments that wind around the cell between the two poles in a cytoskeletal-like structure that appears distinct from a coiled structure formed by the MreB protein. During the proposed grant period we will use a combination of fluorescence microscopy, biochemistrry and genetics to achieve the following aims: i. Min protein structure and function-- To determine the effects of mutations that interfere with the topological specificity function of the MinCDE system, ii. Long-range organization of the Min system-- To define the organization of the MinDE cytoskeletal-like elements within the cell, to determine whether the Min and MreB spiral filaments are separate structures, to characterize proteins that interact with MinD and MinE, and to determine whether there is a relationship between the Min spiral filaments and chromosome segregation, iii. MreB-- To isolate the MreB and Min cytoskeletal-like structures, to characterize proteins that interact with MreB, and to define the behavior of the MreB cytoskeletal-like structures during the course of the cell cycle.

该项目的远程目标是确定细菌选择适当细胞的机制 Midcell的部门。适当地点需要三分钟的蛋白质，即Minc，Mind和Mine 选择。蛋白质具有独特的蜂窝定位模式，并经历了独特的极对极。 振荡周期。我们最近表明，Mincde蛋白被组织成螺旋细丝 在细胞骨架样结构中，两极之间的细胞周围绕，看起来与盘绕的结构不同 由MREB蛋白形成的结构。在拟议的赠款期间，我们将使用 荧光显微镜，生物化学和遗传学以达到以下目的：i。最小蛋白质结构 和功能 - 确定突变的影响 Mincde系统，ii。最小系统的远程组织 - 定义 细胞内的Minde细胞骨架状元素，以确定最小和MREB螺旋细丝是否 是单独的结构，以表征与思想和我相互作用的蛋白质，并确定是否是否 最小螺旋细丝与染色体隔离之间存在关系，iii。 mreb-孤立 MREB和最小细胞骨架样结构，以表征与MREB相互作用的蛋白质，并定义 在细胞周期过程中，MREB细胞骨架样结构的行为。