CONTROL OF TRANSCRIPTION TERMINATION IN E. COLI

大肠杆菌转录终止的控制

• 批准号: 3292398
• 负责人: MAX Elliot GOTTESMAN
• 金额: $ 21.97万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3292398
• 关键词: Escherichia coli; bacterial genetics; gene expression; genetic mapping; genetic transcription; genetic translation; molecular cloning; mutant; nucleic acid sequence; operon; prophages; regulatory gene; ribosomal RNA

Transcription termination on the coliphage lambda chromosome is suppressed by the viral N protein. Host mutations which block N action (nus, groN) also affect transcription termination on the bacterial chromosome. We find that nusB mutants are defective in transcribing ribosomal RNA; we are selecting additional E. coli mutants with this defect. We have designed a novel lambda vector to perform this selection, and to verify that transcription termination is affected in these mutants. Other E. coli mutations that alter transcription termination have been isolated as suppressors of the nusAl phenotype. One such suppressor appears to lie in gene "u", which encodes a 21 kd protein and is located in a cluster of genes involved in mRNA translation. We wish to determine the nature of the "u" product, and to ask if it serves as a link between transcription and translation. Bacteriophage lambda does not propagate on E. coli lysogenic for phage HK022. This exclusion is due to a HK022 prophage function, Nun, which acts as a new transcription termination factor. Nun interacts with Nus factors to provoke premature termination at the lambda nut sites.

抑制了Coliphage Lambda染色体上的转录终止 通过病毒N蛋白。 阻塞N动作的宿主突变（NUS，Gron） 还会影响细菌染色体上的转录终止。 我们发现 NUSB突变体在转录核糖体RNA方面有缺陷。我们是 选择此缺陷的其他大肠杆菌突变体。 我们设计了一个 新颖的lambda矢量来执行此选择，并验证 在这些突变体中，转录终止受到影响。 改变转录终止的其他大肠杆菌突变已经 被隔离为鼻型表型的抑制剂。 一个这样的抑制器 似乎位于基因“ U”，该基因编码21 kd蛋白，位于 参与mRNA翻译的基因簇。 我们希望确定 “ U”产品的性质，并询问它是否充当 转录和翻译。 噬菌体lambda不会在大肠杆菌溶菌蛋白上传播以进行噬菌体 HK022。 此排除是由于HK022预言功能，尼姑 作为新的转录终止因子。 修女与NUS因素相互作用 在Lambda坚果站点提出过早终止。