ROLE OF INTEGRATIVE HOST FACTOR IN GENE EXPRESSION

整合宿主因子在基因表达中的作用

• 批准号: 3290773
• 负责人: MAX Elliot GOTTESMAN
• 金额: $ 22.59万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3290773
• 关键词: Escherichia coli; bacterial genetics; bacterial proteins; bacteriophage lambda; binding proteins; chromosome deletion; endonuclease; gene expression; genetic manipulation; genetic mapping; genetic transcription; genetic translation; messenger RNA; mutant; nucleic acid structure; operon

An E. coli histone-like protein, IHF, acts directly to allow the translation of a Lambda gene, cII. Our hypothesis is that it accomplishes this by altering the structure of the cII transcript so that it becomes accessible to ribosomes; an IHF binding site lies adjacent to the cII Shine-Dalgarno sequence. We wish to analyze this regulatory system by genetic and biochemical approaches. The former is facilitated by our construction of numerous cII gene and operon fusions; the latter by our establishment of coupled transcription-translation system which reflects the IHF requirement for cII expression. We plan also to probe the structure of cII mRNA. Finally, we offer selection protocols to isolate host mutants in which cII expression is IHF-independent; these mutants may display alterations in the other phenotypes caused by an IHF-mutation.

大肠杆菌类组蛋白样蛋白IHF直接起作用以允许 lambda基因的翻译，CII。 我们的假设是它完成了 通过更改CII成绩单的结构，以便它变为 核糖体可进入； IHF结合位点位于CII附近 Shine-Dalgarno序列。 我们希望通过 遗传和生化方法。 前者是由我们的 建造许多CII基因和操纵子融合；后者在我们 建立反映的耦合转录翻译系统 CII表达的IHF要求。 我们还计划调查 CII mRNA的结构。 最后，我们提供选择协议来隔离 CII表达不依赖于IHF的宿主突变体；这些突变体可能 显示由IHF突变引起的其他表型的变化。

项目成果

Repression of the lambda pcin promoter by integrative host factor.

整合宿主因子抑制 lambda pcin 启动子。

• DOI: 10.1016/0022-2836(89)90169-1
• 发表时间: 1989
• 期刊: Journal of molecular biology
• 影响因子: 5.6
• 作者: Griffo,G;Oppenheim,AB;Gottesman,ME
• 通讯作者: Gottesman,ME

Transcription of a bacteriophage lambda DNA site blocks growth of Escherichia coli.

噬菌体 lambda DNA 位点的转录可阻断大肠杆菌的生长。

• DOI: 10.1128/jb.172.2.1030-1034.1990
• 发表时间: 1990
• 期刊: Journal of bacteriology
• 影响因子: 3.2
• 作者: Guzman,P;RiveraChavira,BE;Court,DL;Gottesman,ME;Guarneros,G
• 通讯作者: Guarneros,G