ANS AND TRYPTOPHAN PHOTOPHYSICS: SUPERSONIC JET STUDIES

ANS 和色氨酸光物理学：超音速喷气研究

• 批准号: 3281909
• 负责人: MARK A SULKES
• 金额: $ 13.13万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-05 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281909
• 关键词: bioengineering /biomedical engineering; biophysics; chromophore; conformation; electronic spectra; fluorescence; fluorescence spectrometry; fluorescent dye /probe; gas; laser spectrometry; mass spectrometry; molecular energy level; naphthalenes; photoactivation; solvents; tryptophan; tryptophan analog; vapor; bioengineering /biomedical engineering; biophysics; chromophore; conformation; electronic spectra; fluorescence; fluorescence spectrometry; fluorescent dye /probe; gas; laser spectrometry; mass spectrometry; molecular energy level; naphthalenes; photoactivation; solvents; tryptophan; tryptophan analog; vapor

Study will continue on the effects of partial solvation on the photophysics of important intrinsic and extrinsic fluorescence probe molecules, including principally tryptophan and arylaminonaphthalene sulfonate (ANS) derivatives. Supersonic expansion techniques are used to produce cold, isolated molecule samples of these molecules as well as partially solvated complexes containing various numbers of solvent molecules. The basic rationale of the experiments is to study the photophysics of successively larger solvent complexes in order to learn what specific solvent site interactions or collective solvent structures are necessary to bring about the key photophysical changes observed in bulk solution. In tryptophan further attention must be given to molecular conformation and solvent effects on amino/carboxyl groups while in ANS specific size solvent clusters may be important. As has already been the case, measurement of specific fluorescence lifetimes will yield useful photophysical information. It will be important to measure absorbance spectra as well, by employing a suitable pulsed slit nozzle, and to compare these spectra with laser induced fluorescence spectra for identification of solvent complex bands with quenched emission. The most important now experimental extension is construction of a supersonic jet/molecular beam system, which makes possible the application of photoionization/mass detection methods. By this approach it will be possible first to develop the means of preparing additional biological molecule sample expansions and second to identify and characterize larger solvent complexes.

研究将继续研究部分溶剂化对光体物理学的影响 重要的内在和外在荧光探针分子， 主要是色氨酸和芳基氨基苯二甲酸磺酸盐（ANS） 衍生物。 超音速扩展技术用于产生冷， 这些分子的分离分子样品以及部分溶剂化 包含各种溶剂分子的复合物。 基本 实验的理由是研究依次的光体物理学 较大的溶剂络合物，以了解哪个特定溶剂网站 相互作用或集体溶剂结构是必要的 在散装溶液中观察到的关键光物理变化。 在色氨酸中 必须进一步注意分子构象和溶剂 对氨基/羧基的影响，而在特定尺寸溶剂中 集群可能很重要。 就像已经这样的情况 特定的荧光寿命将产生有用的光物理 信息。 测量吸光度光谱也很重要 通过使用合适的脉冲缝喷嘴，并比较这些光谱 用激光诱导的荧光光谱鉴定溶剂 具有淬火发射的复杂带。 现在最重要的实验 扩展是构造超音速喷气/分子束系统，该系统 使光电离/质量检测方法的应用成为可能。 通过这种方法，将首先开发 准备其他生物分子样品扩展，其次 识别和表征较大的溶剂复合物。