ANS AND TRYPTOPHAN PHOTOPHYSICS: SUPERSONIC JET STUDIES

ANS 和色氨酸光物理学：超音速喷气研究

• 批准号: 2176731
• 负责人: MARK A SULKES
• 金额: $ 12.63万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-05 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2176731
• 关键词: bioengineering /biomedical engineering; biophysics; chromophore; conformation; electronic spectra; fluorescence; fluorescence spectrometry; fluorescent dye /probe; gas; laser spectrometry; mass spectrometry; molecular energy level; naphthalenes; photoactivation; solvents; tryptophan; tryptophan analog; vapor

Study will continue on the effects of partial solvation on the photophysics of important intrinsic and extrinsic fluorescence probe molecules, including principally tryptophan and arylaminonaphthalene sulfonate (ANS) derivatives. Supersonic expansion techniques are used to produce cold, isolated molecule samples of these molecules as well as partially solvated complexes containing various numbers of solvent molecules. The basic rationale of the experiments is to study the photophysics of successively larger solvent complexes in order to learn what specific solvent site interactions or collective solvent structures are necessary to bring about the key photophysical changes observed in bulk solution. In tryptophan further attention must be given to molecular conformation and solvent effects on amino/carboxyl groups while in ANS specific size solvent clusters may be important. As has already been the case, measurement of specific fluorescence lifetimes will yield useful photophysical information. It will be important to measure absorbance spectra as well, by employing a suitable pulsed slit nozzle, and to compare these spectra with laser induced fluorescence spectra for identification of solvent complex bands with quenched emission. The most important now experimental extension is construction of a supersonic jet/molecular beam system, which makes possible the application of photoionization/mass detection methods. By this approach it will be possible first to develop the means of preparing additional biological molecule sample expansions and second to identify and characterize larger solvent complexes.

将继续研究部分溶剂化对光物理的影响 重要的内在和外在荧光探针分子， 主要包括色氨酸和芳基氨基萘磺酸盐 (ANS) 衍生物。 超音速膨胀技术用于产生冷、 这些分子的分离分子样品以及部分溶剂化的 含有不同数量溶剂分子的复合物。 基本的 实验的基本原理是连续研究光物理学 更大的溶剂复合物，以了解特定的溶剂位点 相互作用或集体溶剂结构对于实现 在本体溶液中观察到的关键光物理变化。 色氨酸中 必须进一步注意分子构象和溶剂 在 ANS 特定尺寸溶剂中对氨基/羧基的影响 集群可能很重要。 正如已经发生的情况一样，测量 特定的荧光寿命将产生有用的光物理 信息。 测量吸收光谱也很重要， 通过采用合适的脉冲狭缝喷嘴，并比较这些光谱 用激光诱导荧光光谱来识别溶剂 具有淬灭发射的复杂谱带。 现在最重要的是实验性的 扩展是构建超音速喷射/分子束系统， 使得光电离/质量检测方法的应用成为可能。 通过这种方法，将有可能首先开发出 准备额外的生物分子样品扩展，然后进行 识别和表征较大的溶剂复合物。