PHOTOPHYSICS OF QUANTITATIVE MICROSCOPY FOR LIVING CELLS

活细胞定量显微镜的光物理学

• 批准号: 3421753
• 负责人: WATT W WEBB
• 金额: $ 21.56万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-06-05 至 1997-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3421753
• 关键词: benzopyrans; bioengineering /biomedical engineering; biophysics; cell biology; cellular pathology; chromophore; computer program /software; confocal scanning microscopy; diffusion; fluoresceins; fluorescence; fluorescent dye /probe; image processing; ionophores; lasers; optics; photobiology; photochemistry; photolysis

DESCRIPTION: (Adapted from Applicant's Abstract) The creation of a powerful new non-linear laser microscopy for biomedical research and pathology investigations is the objective of this research. New and convenient instrumentation for imaging the dynamic microchemistry of individual living cells and tissues is to be developed from the recent invention: "Two Photon Excitation Laser Scanning Fluorescence Microscopy" (Denk, Strickler and Webb, Science 248:73-76,1990). Specific projects include measurement of two photon excitation spectra of fluorophores, engineering design for the non-linear optical system including adaptation of economical solid state femtosecond lasers and integration of powerful analytic control computers, development of new methods of measurement of three-dimensional molecular diffusion (as in the cellular cytoplasm) enabled by the inherent three-dimensional resolution of two-photon excitation and photolysis, measure molecular mechanisms of photolysis, photobleaching and cellular photodamage with the help of high intensity laser pulses, and develop imaging of fluorescence decay time data for cellular microenvironmental analysis. Introduction of non-linear physical optics into optical microscopy offers an opportunity for major advance in analytical capability for quantitative biophysical instrumentation that this research aims to realize.

描述：（改编自申请人的摘要） 创建强大的新型非线性激光显微镜 生物医学研究和病理学研究是 本研究的目的。 又新又方便 用于动态微化学成像的仪器 个体活细胞和组织是由 最近的发明：“双光子激发激光扫描 荧光显微镜”（Denk、Strickler 和 Webb，《科学》 248：73-76，1990）。 具体项目包括测量 荧光团的双光子激发光谱，工程 非线性光学系统的设计，包括适配 经济型固态飞秒激光器及其集成 强大的分析控制计算机，新的开发 三维分子测量方法 扩散（如在细胞质中）由 双光子固有的三维分辨率 激发和光解，测量分子机制 光解、光漂白和细胞光损伤 高强度激光脉冲的帮助，并开发成像 细胞微环境的荧光衰减时间数据 分析。将非线性物理光学引入 光学显微镜为重大进展提供了机会 定量生物物理分析能力 本研究旨在实现的仪器。