CONSULTATIONS ON APPLICATIONS OF MULTI PHOTON MICROSCOPY

多光子显微镜应用咨询

• 批准号: 6349480
• 负责人: WATT W WEBB
• 金额: $ 0.11万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349480
• 关键词: bioengineering /biomedical engineering; biomedical resource; education; technology /technique

A technique for the measurement of the two-photon uncaging action cross section of calcium cages has been developed in this laboratory $^{1,2}$. Using this technique, we report on the prognosis for effective two-photon excitation of a novel calcium cage, DMNPE-4 $^{3}$. We also describe calculations which predict the total amount of calcium ions uncaged and the temporal behavior of the liberated calcium distribution under given multiphoton photolysis conditions with a given cage. These calculations, combined with the two-photon uncaging action cross sections that we have measured for a variety of calcium cages enable the quantitative study of calcium dependent processes with high spatial resolution within cells or thick tissue samples. The ultimate intent of these studies is the application of these tools to the quantitative study of the spontaneous transient calcium concentration elevations ("sparks") in developing skeletal muscle cells, probing the potentiation/inhibition relation ships between consecutive sparks in one localized region as well as between sparks in spatially separate regions. 1. Shear, J. et al. {\em Biophys. J.}70(2) A211 (1996) 2. Brown et al, Manuscript in Preparation (1997) 3. Ellis-Davies, G.{\em Biophys. J.}72(2) A246 (1997)

一种测量两光子分离动作的技术 该实验室已经开发了钙笼的横截面 $^{1,2} $。 使用此技术，我们报告了预后 新型钙笼的有效两光子激发DMNPE-4 $^{3} $。 我们还描述了预测总量的计算 钙离子未分解和解放的时间行为 在给定多光子光解条件下的钙分布 带有给定的笼子。 这些计算，结合了两光子 我们已经为各种衡量的分离动作横截面 钙笼可以实现钙依赖性的定量研究 细胞或厚组织内空间分辨率高的过程 样品。 这些研究的最终目的是应用 这些工具用于自发瞬态的定量研究 钙浓度高程（“火花”）在发展骨骼中 肌肉细胞，探测增强/抑制关系。 在一个本地区域以及之间的连续火花之间 在空间分开的区域中的火花。 1。Shear，J。等。 {\ em 生物。 J.} 70（2）A211（1996）2。Brown等，手稿 制备（1997）3。 J.} 72（2）A246 （1997）