ANS AND TRYPTOPHAN AND PHOTOPHYSICS: SUPERSONIC JET STU

ANS 和色氨酸和光物理学：超音速喷射 STU

• 批准号: 3281907
• 负责人: MARK A SULKES
• 金额: $ 10.17万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-05 至 1988-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3281907
• 关键词: conformation; electronic spectra; fluorescence spectrometry; fluorescent dye /probe; laser spectrometry; mathematical model; naphthalenes; phase change; photochemistry; tryptophan; tryptophan analog; water solution; conformation; electronic spectra; fluorescence spectrometry; fluorescent dye /probe; laser spectrometry; mathematical model; naphthalenes; phase change; photochemistry; tryptophan; tryptophan analog; water solution

The photophysics of arylaminonaphthalene sulfonate (ANS) and tryptophan molecules, in bare molecule and partially solvated forms, will be studied. Supersonic expansion techniques will be used to prepare the samples and laser spectroscopic techniques employed to study them. The principal methods of spectroscopy to be used are pulsed, tunable laser fluorescence excitiaton scans; dispersed fluoresecence of specific features excited; and fluorescence lifetime measurements of specific excited state features, the latter carried out using a synchronously pumped dye laser and time-correlated photon counting. In biological systems ANS derivatives can be used as extrinsic fluorescence probes and tryptophan as a intrinsic one. The photophysics of these molecules is not, however, fully understood. In both cases there have been experimental advances in recent years, primarily involving lifetime measurements in solution environments, and photophysical hypotheses have been refined for each class of molecules. In ANS-type molecules a charge transfer state, facilitated by solvents such as water, appears to be of primary importance; in tryptophan, important effects might arise from molecular conformation, energetically close excited states, and solvent effects on the amino and carboxyl groups. Supersonic expansions can address questions on a molecular scale of interaction; condensed phase experiments cannot offer this possibility. First, the photophysics of bare single molecules will be studied. In particular, control of rotameric conformation of bare tryptophan derivatives should be possible, a potentially powerful tool in studying tryptophan photophysics. Next, ANS and tryptophan derivatives will be complexed with selected solvent molecules, such as water. Spectroscopic bands from different sized complexes will be identified and selectively laser excited for studies of emission properties. In the process of carrying out this work, refinements will be attempted for resolving and characterizing spectroscopic bands from partially solvated complexes with larger numbers of addends. These advances will enable additional solvation studies to proceed on ANS and tryptophan, as well as on other biophysically interesting molecules. The long-term objective is to develop and confirm a complete picture of the photophysics of these systems. It is hoped that, with such an understanding, the influence of environment and bonding on the fluorescent probes' responses can be given a more detailed and correct interpretation.

芳基氨基苯二甲酸盐（ANS）和色氨酸的光物理学 将研究以裸分子和部分溶剂化的形式进行的分子。 超音速扩展技术将用于准备样品和 用于研究它们的激光光谱技术。 校长 要使用的光谱法的方法是脉冲，可调激光荧光的 激发扫描；激发特定特征的荧光分散；和 特定激发态特征的荧光寿命测量， 后者使用同步泵送的染料激光进行 时间相关的光子计数。 在生物系统中，ANS衍生物可以 用作外在荧光探针和色氨酸作为固有的 一。 但是，这些分子的光物理并非完全 理解。 在这两种情况下，最近都有实验性进步 几年，主要涉及解决方案环境中的终身测量值 和光物理假设已针对每类 分子。 在ANS型分子中，电荷转移态，由 诸如水之类的溶剂似乎至关重要。在色氨酸中， 重要的效果可能是由于分子构象而产生的 关闭激发态，以及对氨基和羧基的溶剂作用 组。 超音速扩展可以解决分子规模的问题 相互作用；冷凝相实验无法提供这种可能性。 首先，将研究裸露的单分子的光体物理学。 在 特别，控制裸色氨酸的旋转构象 衍生物应该是可能的，这是研究的潜在强大工具 色氨酸光物理学。 接下来，ANS和色氨酸衍生物将是 与选定的溶剂分子（例如水）复合。 光谱学 将确定和选择性地识别来自不同大小配合物的频段 激发了对发射特性的研究。 在 进行这项工作，将尝试进行改进，以解决和 表征来自部分溶剂复合物的光谱带与 更多的加成。 这些进步将实现其他溶剂化 在ANS和色氨酸以及其他生物物理上进行的研究 有趣的分子。 长期目标是开发和确认 这些系统的光体物理学的完整图片。 希望， 有了这样的理解，环境的影响和纽带对 荧光探针的响应可以得到更详细和更正确的 解释。