TRANSIENT INFRARED SPECTROSCOPIC STUDIES OF PROTEIN DYNAMICS

蛋白质动力学的瞬态红外光谱研究

• 批准号: 6204244
• 负责人: ROBIN Main HOCHSTRASSER
• 金额: $ 14.76万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6204244
• 关键词: chemical stability; cofactor; conformation; cytochrome c; electric field; electron transport; electronic spectra; fluorescence spectrometry; infrared spectrometry; ligands; molecular dynamics; photochemistry; photosynthetic reaction centers; protein structure function; synthetic protein; time resolved data; tryptophan

The research is aimed at characterizing the responses of various media to optically triggered events. The triggering involves electronic excitation, electron transfer, photochemical reaction and ligand release. The various media include model solvents and proteins. The specific goal is to learn how proteins respond to changes in the structure or charge distribution of chromophores imbedded in them or to charge separation. The novel method of transient infrared spectroscopy will be used in order that the vibrational frequencies and dynamics of the equilibrium distribution can be evaluated. Specifically, experiments will be carried out on electron transfer processes in reaction centers from photosynthetic bacteria and model systems in which the incoherent and coherent responses will be explored. Ligand release experiments on hemoglobin and myoglobin are planned to investigate the resulting protein conformational change. Also the structural responses of the 'de novo' synthesized proteins will be studied by infrared methods. Efforts are underway to devise new methods of understanding the IR spectra of proteins using hole burning.

该研究旨在描述不同媒体对 光学触发事件。触发涉及电子激励， 电子转移、光化学反应和配体释放。各种 介质包括模型溶剂和蛋白质。具体目标是学习 蛋白质如何响应结构或电荷分布的变化 嵌入其中的发色团或电荷分离。新颖的方法是 将使用瞬态红外光谱，以便振动 可以评估平衡分布的频率和动态。 具体来说，将进行电子转移实验 光合细菌反应中心的过程和模型 将探索不连贯和连贯响应的系统。 计划进行血红蛋白和肌红蛋白的配体释放实验 研究由此产生的蛋白质构象变化。还有 将研究“从头”合成蛋白质的结构反应 通过红外方法。目前正在努力设计新方法 使用烧孔了解蛋白质的红外光谱。