TRANSPORT ACROSS BIOLOGICAL MEMBRANES

跨生物膜的运输

• 批准号: 3271429
• 负责人: JEREMY R KNOWLES
• 金额: $ 7.21万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-04-01 至 1990-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3271429
• 关键词: Escherichia coli; autoradiography; bacterial genetics; bacteriophage M13; beta lactamase; conformation; gene expression; gene mutation; genetic library; genetic manipulation; genetic promoter element; genetic recombination; gram negative bacteria; membrane model; membrane permeability; membrane proteins; membrane structure; mutant; nucleic acid sequence; oligopeptides; photochemistry; plasmids; protein signal sequence; protein transport; radiation genetics; radiotracer; tissue /cell culture

The proposals outlined relate to three different aspects of transport across biological membranes. a. How are proteins that are destined for locations other than the cytoplasmic site of their synthesis, 'exported' across the membrane boundary? Methods are being developed to mutate the signal sequence of E. coli beta-lactamase by in vitro chemical mutagenesis of a segment of the gene that includes the signal sequence of this enzyme. b. What is the precise labeling pattern produced when a transmembrane protein, the coat protein from the bacteriophage M13, is photo-labeled by the carbene derived from (3H)adamanthyl diazirine? c. What is the scope of the 'illicit transport' approach to the smuggling of relatively small foreign molecules into a bacterial cell? The extent to which the oligopeptide transport system can be used for illicit transport will be delineated, with a view both to experiments concerning the delivery of antibiotics, and to experiments probing the distribution of membrane proteins as seen from the cytoplasmic face of the membrane.

概述的提议与遍历运输的三个不同方面有关 生物膜。 一个。 蛋白质的注定位置如何 除了其合成的细胞质部位， 膜边界？ 正在开发方法来突变信号序列 大肠杆菌β-内酰胺酶通过体外化学诱变的基因段 这包括该酶的信号序列。 b。 什么是确切的 当跨膜蛋白（从 噬菌体M13是由源自源自的卡宾标记的 （3h）adamanthyl dizirine？ c。 “非法运输”的范围是什么 将相对较小的外国分子走私到细菌的方法 细胞？ 寡肽传输系统的使用程度可用于 非法运输将被划定，以期有关 抗生素的递送，以及探测分布的实验 从膜的细胞质面看，膜蛋白。

项目成果

The role of the beta-lactamase signal sequence in the secretion of proteins by Escherichia coli.

β-内酰胺酶信号序列在大肠杆菌分泌蛋白质中的作用。

• 发表时间: 1984
• 期刊: The Journal of biological chemistry
• 作者: Kadonaga,JT;Gautier,AE;Straus,DR;Charles,AD;Edge,MD;Knowles,JR
• 通讯作者: Knowles,JR

Targeted point mutation that creates a unique Eco RI site within the signal codons of the beta-lactamase gene without altering enzyme secretion or processing.

靶向点突变可在 β-内酰胺酶基因的信号密码子内创建独特的 Eco RI 位点，而不改变酶的分泌或加工。

• 发表时间: 1982
• 期刊: The Journal of biological chemistry
• 作者: Charles,AD;Gautier,AE;Edge,MD;Knowles,JR
• 通讯作者: Knowles,JR