RETINAL MICROCIRCUITRY--STRUCTURE AND FUNCTION

视网膜微电路——结构和功能

• 批准号: 3264771
• 负责人: KEN-ICHI NAKA
• 金额: $ 21.47万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3264771
• 关键词: amacrine cells; axon; catfish; cone cell; electron microscopy; fresh water environment; histochemistry /cytochemistry; luminescence; neural information processing; neural transmission; neuroanatomy; neurophysiology; optic nerve; photostimulus; retinal ganglion; stimulus /response; synapses; visual feedback; visual photoreceptor

The final goal of this research, which began in 1968, is to produce a well-defined functional morphology of the vertebrate retina. We have developed a comprehensive methodology based on Wiener's theory of nonlinear analysis (white-noise analysis) and have accumulated a large body of data that characterize the morphology and physiology, including the response dynamics of neurons in the retina. The principal preparation used is the retina of the channel catfish. This current research proposal has three major aims: (1) Identification of neuronal circuitry in the inner retina. This is accomplished by injecting current into one neuron and by recording the evoked response, spike discharges or intracellular potential, form another neuron located nearby. Transfer functions will be obtained by a cross-correlation process. We expect to characterize three modes of transmission: very fast transmission mediated by electric coupling, fast (probably mono- synaptic) transmission and slow poly-synaptic transmission. (2) The functional connections will be referenced to the synaptic organization of the participating cells by injecting them with HRP and conducting electron microscopic observations of the nature and distribution of their synaptic connections. (3) Receptive fields. Neurons in the retina form a receptive field that changes in time and space. We will use three measures to explore receptive-field organization: (a) conventional stimuli consisting of either a combination of spot/annulus, drifting sinusoids, or a sweeping bar of light, (b) two input white-noise experiments to define dynamics of the receptive field center and surround, and (c) one-dimensional drifting white-noise grating. The results will be referenced against the neuronal connections in catfish retina. These three lines of inquiry will provide the basis for the development of a comprehensive view of the functional organization of the (cone-driven) neuron network in the inner retina.

这项研究始于1968年的最终目标是生产 脊椎动物视网膜的定义明确的功能形态。 我们已经基于 维也纳的非线性分析理论（白噪声分析）和 积累了大量的数据，这些数据表征 形态和生理学，包括的反应动力学 视网膜中的神经元。 所使用的主要准备工作是视网膜 频道cat鱼。 当前的研究建议有三个 主要目的： （1）鉴定内部视网膜中神经元电路。 这是 通过将电流注入一个神经元和 记录诱发的响应，尖峰放电或细胞内 潜力，形成附近的另一个神经元。 转移 功能将通过互相关过程获得。 我们 期望表征三种传输模式：非常快 通过电耦合介导的变速器，快速（可能是单声道 突触）传输和慢速多突触传播。 （2）功能连接将参考突触 通过向HRP注入参与细胞的组织 并进行电子显微镜观察自然观察 并分布其突触连接。 （3）接受场。 视网膜中的神经元形成一个接受场 这会改变时间和空间。 我们将采取三项措施 探索接收场组织：（a）常规刺激 由斑点/环的组合组合，漂流 正弦或一杆光线，（b）两个输入白噪声 定义接收场中心的动力学的实验和 周围和（c）一维漂流的白色噪声光栅。 结果将参考针对的神经元连接 cat鱼视网膜。 这三条询问将为 发展功能的全面观点 内部（锥体驱动）神经元网络的组织 视网膜。