RETINAL MICROCIRCUITRY--STRUCTURE AND FUNCTION

视网膜微电路——结构和功能

• 批准号: 3264773
• 负责人: KEN-ICHI NAKA
• 金额: $ 20.25万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-08-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3264773
• 关键词: amacrine cells; axon; catfish; cone cell; electron microscopy; fresh water environment; histochemistry /cytochemistry; luminescence; neural information processing; neural transmission; neuroanatomy; neurophysiology; optic nerve; photostimulus; retinal ganglion; stimulus /response; synapses; visual feedback; visual photoreceptor

The final goal of this research, which began in 1968, is to produce a well-defined functional morphology of the vertebrate retina. We have developed a comprehensive methodology based on Wiener's theory of nonlinear analysis (white-noise analysis) and have accumulated a large body of data that characterize the morphology and physiology, including the response dynamics of neurons in the retina. The principal preparation used is the retina of the channel catfish. This current research proposal has three major aims: (1) Identification of neuronal circuitry in the inner retina. This is accomplished by injecting current into one neuron and by recording the evoked response, spike discharges or intracellular potential, form another neuron located nearby. Transfer functions will be obtained by a cross-correlation process. We expect to characterize three modes of transmission: very fast transmission mediated by electric coupling, fast (probably mono- synaptic) transmission and slow poly-synaptic transmission. (2) The functional connections will be referenced to the synaptic organization of the participating cells by injecting them with HRP and conducting electron microscopic observations of the nature and distribution of their synaptic connections. (3) Receptive fields. Neurons in the retina form a receptive field that changes in time and space. We will use three measures to explore receptive-field organization: (a) conventional stimuli consisting of either a combination of spot/annulus, drifting sinusoids, or a sweeping bar of light, (b) two input white-noise experiments to define dynamics of the receptive field center and surround, and (c) one-dimensional drifting white-noise grating. The results will be referenced against the neuronal connections in catfish retina. These three lines of inquiry will provide the basis for the development of a comprehensive view of the functional organization of the (cone-driven) neuron network in the inner retina.

这项研究始于 1968 年，最终目标是生产 脊椎动物视网膜的明确功能形态。 我们开发了一套全面的方法论 维纳非线性分析理论（白噪声分析）和 积累了大量的数据来表征 形态学和生理学，包括反应动力学 视网膜中的神经元。 使用的主要制剂是视网膜 斑点叉尾鮰的。 目前的研究计划有三个 主要目标： (1) 视网膜内层神经元回路的识别。 这是 通过将电流注入一个神经元并通过 记录诱发反应、尖峰放电或细胞内 潜力，形成位于附近的另一个神经元。 转移 函数将通过互相关过程获得。 我们 期望表征三种传输模式： 非常快 由电耦合介导的传输，快速（可能是单 突触）传递和慢速多突触传递。 (2) 功能连接将参考突触 通过向参与细胞注射 HRP 来组织它们 并对自然界进行电子显微镜观察 及其突触连接的分布。 (3)感受野。 视网膜中的神经元形成感受野 随时间和空间的变化。 我们将采取三项措施 探索感受野组织：(a) 常规刺激 由点/环、漂移的组合组成 正弦曲线，或扫光条，(b) 两个输入白噪声 定义感受野中心动态的实验 环绕，以及（c）一维漂移白噪声光栅。 结果将参考神经元连接 鲶鱼的视网膜。 这三方面的调查将为以下问题提供基础： 发展功能的综合视图 内部（视锥驱动）神经元网络的组织 视网膜。