REGULATION OF FOOD INTAKE IN CROSSED INTESTINES RATS

交叉肠大鼠摄食的调节

• 批准号: 3246790
• 负责人: HENRY S KOOPMANS
• 金额: $ 4.07万
• 依托单位: UNIVERSITY OF CALGARY
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-30 至 1995-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3246790
• 关键词: appetite; bioenergetics; blood chemistry; brown fat; gastrointestinal absorption /transport; gastrointestinal function; gastrointestinal motility /pressure; gastrointestinal surgery; hormone regulation /control mechanism; laboratory rat; nutrient intake activity; nutrition related tag; oxygen consumption; stomach emptying; sympathetic nervous system; thyroid hormones

The crossed intestines surgery in parabiotic rats produces large changes in daily food intake and energy expenditure that appear to be sustained for the rest of the animal's lives. One rat in each pair can eat three to four times as much as its partner. A major objective of this grant proposal is to determine how these large changes in daily food intake will affect gastric distension, gastric emptying and intestinal transit in order to understand the role of the gastrointestinal tract in the control of daily intake. These experiments will determine which sections of the gastrointestinal tract are stimulated by the food eaten by these rats throughout the 17 hour feeding period. Another objective is to extend pilot studies that show that there are also large changes in oxygen consumption and sympathetic nervous system activity in crossed intestines rats. The possible mechanisms for the changes in overall energy expenditure will be assessed by measuring the rate of norepinephrine turnover, the stimulation of hepatic mRNA levels by thyroid hormones and the amount of uncoupling protein in brown adipose tissue. The final objective is to determine how these large changes in food intake and energy expenditure could be caused by changes in the plasma levels of various hormones and metabolites. The major plasma nutrients, glucose, lactate, glycerol, triglycerides and free fatty acids, will be measured in these rats at several times during the 17 hour feeding period and the plasma level of three pancreatic hormones, insulin, glucagon and somatostatin, will be determined. The long-term objective of this research is to find a medical treatment (drug or hormone analog) for obesity. In Western societies, obesity is a major medical problem that causes a great deal of human suffering. It is associated with such chronic and debilitating conditions as diabetes, hypertension, stroke and heart disease. An effective medical treatment for obesity would improve the quality of life for millions of people and would greatly reduce the cost of long-term health care.

对抗生素大鼠的交叉肠道手术会产生巨大的变化 在每日食物摄入和能量支出中 在动物的余生中。每对一对一只老鼠可以吃三到 是其合作伙伴的四倍。 这笔赠款的主要目标 提案是确定这些日常食物摄入量的这些大变化 会影响胃扩张，胃排空和肠道 为了了解胃肠道在 控制日常摄入量。 这些实验将确定哪些部分 胃肠道的食物被这些食物刺激 在整个17小时喂养期间。 另一个目标是 扩展了试点研究，表明也有很大变化 交叉中的氧气消耗和交感神经系统活动 肠道。 总体变化的可能机制 能源支出将通过测量速度来评估 去甲肾上腺素的周转率，刺激肝mRNA水平 甲状腺激素和棕色脂肪中的解偶联蛋白的量 组织。 最终目标是确定这些大型变化如何 食物摄入和能量消耗可能是由变化引起的 各种激素和代谢产物的血浆水平。 主要等离子体 营养，葡萄糖，乳酸，甘油，甘油三酸酯和游离脂肪 酸将在17小时内几次在这些大鼠中测量 进食期和三种胰腺激素的等离子水平， 将确定胰岛素，胰高血糖素和生长抑素。 长期 这项研究的目的是找到治疗（药物或 激素类似物）肥胖。 在西方社会，肥胖是主要的 造成大量人类苦难的医学问题。 这是 与糖尿病这样的慢性和衰弱状况相关， 高血压，中风和心脏病。 有效的医疗 因为肥胖将改善数百万人的生活质量，并且 将大大降低长期医疗保健的成本。