CAUDAL BRAINSTEM CONTROL OF ENERGY HOMEOSTASIS

尾部脑干对能量稳态的控制

• 批准号: 3409865
• 负责人: FRANCIS W FLYNN
• 金额: $ 9.98万
• 依托单位: UNIVERSITY OF WYOMING
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-09-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3409865
• 关键词: afferent nerve; appetite regulatory center; avoidance behavior; behavior test; bioenergetics; blood chemistry; bombesin; brain stem; cholecystokinin; eating; efferent nerve; experimental brain lesion; homeostasis; laboratory rat; neural information processing; neuropsychology; nutrition related tag; psychophysiology; sympathetic nervous system; taste

Evidence supports a role of gut and brain peptides, functioning as neurotransmitters and neuromodulators, in the control of energy homeostatic responses. Bombesin (BBS) is one such peptide and high concentrations of BBS binding sites are present in the gastrointestinal tract and caudal brainstem (CBS). Furthermore, converging lines of evidence support the notion that BBS participates in normal satiation. The effects of BBS injection on feeding appear to mediated via both ascending visceral afferent signals and this direct action on brain sites. Recent reports from this lab indicate that the caudal brainstem is a site where humoral BBS acts to inhibit feeding. This application describes experiments that will examine the physiological role of brainstem BBS in the control of food intake and satiety, the neural and sensory mechanisms that mediate 4th ventricle bombesin's effect on food intake, and the CBS sites of BBS action. First, what is the functional relationship between 4th icv BBS and peripheral peptide injection (cholecystokinin (CCK8), BBS) effects on feeding? Measurements of food intake following combined 4th icv BBS and systemic BBS and CCK injections will indicate if these signals interact (synergistically or additively) or activate independent neural systems. An interaction would provide evidence for a gut- brainstem peptide axis in the control of food intake. Second, the relationship between BBS-elicited afferent signals originating in the CBS and other levels of the neuraxis and periphery will be examined. Results obtained from decerebrate rats and rats with peripheral efferent denervation will show whether 4th icv BBS injection effects are mediated by neural interactions with forebrain and/or peripheral sites. Third, direct injections of BBS into CBS areas with high concentrations of BBS binding sites will demonstrate the functional significance of these sites in the control of food intake. Last, the sensory consequences (satiety, malaise) of 4th icv BBS injection will be inferred by measuring the behavioral specificity of 4th icv bombesin's inhibitory effects and whether rats developed a taste aversion to stimuli associated with 4th icv BBS injection. When taken together, the results will demonstrate the functional significance of CBS injection. When taken together, the results will demonstrate the functional significance of CBS afferent signals elicited by BBS in the control of food intake. The information gained will further decipher the organization of ingestive behavior and gut-brain peptide interactions in the control of energy homeostasis.

证据支持肠道和脑肽的作用，起作用 神经递质和神经调节剂，控制能量 稳态反应。 孟买（BBS）就是这样的肽 BBS结合位点的浓度存在于 胃肠道和尾脑干（CBS）。 此外， 融合的证据线支持BBS 参加正常的饱腹感。 BBS注入对 喂养似乎是通过两个上升的内脏传入介导的 信号和对大脑部位的直接作用。 最近的报告 该实验室表明，尾脑干是一个体液的部位 BB的作用是抑制喂养。 此应用程序描述 将检查脑干的生理作用的实验 BB控制食物摄入和饱腹感，神经和 感官机制介导第四心室孟买对 食物摄入量和BBS动作的CBS站点。 首先，什么是 第四次ICV BBS和外围肽之间的功能关系 注射（胆囊菌（CCK8），BBS）对进食的影响？ 第4次ICV BBS和 全身性BB和CCK注射将指示这些信号是否 互动（协同或附加性）或激活独立 神经系统。 互动将为肠道提供证据 脑干肽轴控制食物摄入量。 第二， 源自BBS引用的传入信号之间的关系 CBS和其他层次的神经和外围将是 检查。 从杂交大鼠和大鼠的结果获得的结果 外围传出的神经支配将显示第四次ICV BBS是否 注射效应是由神经相互作用与 前脑和/或外围部位。 第三，直接注射BBS 进入高浓度BBS结合位点的CBS区域 证明这些位点在 控制食物摄入量。 最后，感官后果（饱腹感， 通过测量 第四届ICV Bombesin抑制作用的行为特异性 以及大鼠是否形成了与刺激相关的味道厌恶 与第四次ICV BBS注入。 当一起，结果将 证明CBS注射的功能意义。 什么时候 综上所述，结果将证明功能 BBS在控制中引起的CBS传入信号的重要性 食物摄入量。 获得的信息将进一步破译 摄入行为和肠道肽的组织 控制能量稳态的相互作用。