Towards on-column monitoring of protein purification by ATR-FTIR and Raman Spectroscopy

通过 ATR-FTIR 和拉曼光谱对蛋白质纯化进行柱上监测

• 批准号: BB/R019533/1
• 负责人: Bernadette Byrne
• 金额: $ 25.39万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR019533%2F1
• 关键词: Towards; column; monitoring; protein; purification

Biopharmaceuticals, proteins used as drugs, are an emerging area in the treatment of a range of diseases including rheumatoid arthritis and cancer. While these therapeutics have been shown to be very effective, they are also extremely expensive (~£10,000s/treatment), due to the complex, large scale production processes required. The most expensive step is Protein A affinity capture. Previous research by our group has utilised a specialized technique called Attenuated Total Reflectance-Fourier Transform Infrared (ATR-FTIR) spectroscopy to explore the causes of Protein A resin decay. The research outlined in this proposal aims to further characterise binding capacity decay using this approach and also develop the approach to make this a tool that will be useful to scientists in an industrial bioprocessing environment. Our direct monitoring approach will provide much greater control into Protein A purification and has the potential to extend resin life time, saving production costs.

生物药物，用作药物的蛋白质是治疗包括类风湿关节炎和癌症在内的各种疾病的新兴领域。尽管这些治疗剂已被证明非常有效，但由于需要复杂的大规模生产过程，它们也非常昂贵（约10,000英镑/治疗）。最昂贵的步骤是蛋白质A亲和力捕获。我们小组的先前研究利用了一种专门的技术，称为衰减的总反射率转换红外（ATR-FTIR）光谱法来探索蛋白A树脂衰减的原因。该提案中概述的研究旨在使用这种方法进一步表征约束能力衰减，并开发方法使该工具成为工业生物处理环境中对科学家有用的工具。我们的直接监测方法将为蛋白质A纯化提供更大的控制，并有可能延长树脂寿命，从而节省生产成本。

项目成果

Vibrational Spectroscopy in Protein Research

蛋白质研究中的振动光谱学

• 发表时间: 2020
• 作者: Byrne, B

Insight into purification of monoclonal antibodies in industrial columns via studies of Protein A binding capacity by in situ ATR-FTIR spectroscopy.

• DOI: 10.1039/d1an00985k
• 发表时间: 2021-08-09
• 期刊: The Analyst
• 作者: Beattie JW;Rowland-Jones RC;Farys M;Tran R;Kazarian SG;Byrne B
• 通讯作者: Byrne B

Application of Raman Spectroscopy to Dynamic Binding Capacity Analysis.

• DOI: 10.1177/00037028231210293
• 发表时间: 2023-12
• 期刊: Applied spectroscopy
• 影响因子: 3.5

Causes of Industrial Protein A Column Degradation, Explored Using Raman Spectroscopy.

• DOI: 10.1021/acs.analchem.2c03063
• 发表时间: 2022-11-15
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Beattie JW;Istrate A;Lu A;Marshall C;Rowland-Jones RC;Farys M;Kazarian SG;Byrne B
• 通讯作者: Byrne B

sj-docx-1-asp-10.1177_00037028231210293 - Supplemental material for Application of Raman Spectroscopy to Dynamic Binding Capacity Analysis

sj-docx-1-asp-10.1177_00037028231210293 - 拉曼光谱在动态结合能力分析中应用的补充材料

• DOI: 10.25384/sage.24487412
• 发表时间: 2023
• 作者: Beattie J