K-562: A HUMAN PLURIPOTENT LEUKEMIA STEM CELL LINE

K-562：人类多能白血病干细胞系

• 批准号: 3170039
• 负责人: CARMEN BERTUCCI LOZZIO
• 金额: $ 17.61万
• 依托单位: UNIVERSITY OF TENNESSEE KNOXVILLE
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-05-01 至 1987-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3170039
• 关键词: T lymphocyte; cell differentiation; chromosome disorders; chromosome translocation; cytogenetics; erythrocytes; gene expression; genetic markers; granulocyte; hematopoietic stem cells; human tissue; immunohematology; karyotype; leukemia; lymphocyte; megakaryocytes; monoclonal antibody; monocyte; neoplasm /cancer remission /regression; neoplastic cell; neoplastic growth; peroxidation; tissue /cell culture; tumor antigens

The human multipotential stem cell line K-562 is an excellent model for the study of cell differentiation in human leukemia cells. This cell line was originally established at our laboratory with blasts from a patient with chronic myelocytic leukemia (CML) in terminal blast crisis. The original line has been maintained in serial culture for 14 years and many sublines have been derived from samples of cells frozen at various passages. The K-562 cells are highly undifferentiated blasts with the potential for differentiation along the erythrocytic, granulocytic, monocytic, lymphocytic, and megakaryocytic cell lineages. Each subline has characteristic chromosomal aberrations and antigenic expression for a variety of hematopoietic differentiation antigens. The specific aims of this project are: 1) to study the morphologic features and changes in antigenic expression induced by biological response modifiers or inducers of cell differentiation such as hemin, dimethysulfoxide, retinoic acid, DNA inhibitors, growth factors, interleukins 1 and 2 and specific antibodies; 2) to assess if the K-562 cells lose their characteristic malignancy at some stage of differentiation and maturation; 3) to investigate correlations between specific chromosome aberrations and the response of the cells to various inducers; and 4) to study the relationship between oncogene activation at the breaking points of chromosomal aberrations and the potential for differentiation of different sublines. The results of the studies will help elucidate the mechanism involved in the arrest of cell differentiation present in highly malignant blasts. This information will increase our understanding on how malignant cells can be induced to differentiate and to stop or reduce their active proliferation. The long term goal is to develop new therapeutic modalities for myelogenous, lymphoid or erythroid types of human leukemia. (M)

人类多能干细胞系K-562是一个优秀的模型 人类白血病细胞的细胞分化研究。 该细胞系是 最初是在我们的实验室用来自患有以下疾病的患者的原始细胞建立的 慢性粒细胞白血病（CML）处于末期急变期。 原来的 该品系已在系列文化中维持了 14 年，并有许多子品系 源自不同传代的冷冻细胞样本。 这 K-562 细胞是高度未分化的母细胞，具有潜在的 沿红细胞、粒细胞、单核细胞分化， 淋巴细胞和巨核细胞谱系。 每个子线都有 特征性染色体畸变和抗原表达 多种造血分化抗原。 具体目标 该项目的目的是：1）研究形态特征和变化 生物反应调节剂或诱导剂诱导的抗原表达 细胞分化，如氯高铁血红素、二甲亚砜、视黄酸、DNA 抑制剂、生长因子、白细胞介素1和2以及特异性抗体； 2) 评估 K-562 细胞是否在 分化和成熟的某个阶段； 3）调查 特定染色体畸变与反应之间的相关性 细胞对各种诱导剂的作用； 4）研究之间的关系 在染色体畸变的临界点激活癌基因 不同亚系的分化潜力。 结果 这些研究将有助于阐明逮捕的机制 高度恶性的原始细胞中存在细胞分化。 此信息 将增加我们对恶性细胞如何被诱导的理解 区分并阻止或减少它们的活跃增殖。 长的 长期目标是开发新的治疗方式来治疗骨髓性、 人类白血病的淋巴或红细胞类型。 (男)