PATHOGENETIC DETERMINANTS OF HUMAN CNS TUMORS

人类中枢神经系统肿瘤的致病决定因素

• 批准号: 3164069
• 负责人: laura MANUELIDIS
• 金额: $ 17.53万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-12-01 至 1989-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3164069
• 关键词: DNA; autoradiography; brain neoplasms; carcinogens; cell sorting; cell type; central nervous system neoplasms; chemical carcinogenesis; chemical fingerprinting; chromatin; chromosomes; clone cells; computer data analysis; cytogenetics; electron microscopy; embryo neoplasm; fluorescence microscopy; fluorescent dye /probe; fluorimetry; genetic mapping; glioma; glycoproteins; guinea pigs; hamsters; histochemistry /cytochemistry; human tissue; immunogenetics; in situ hybridization; laboratory mouse; monoclonal antibody; neoplasm /cancer genetics; nucleic acid denaturation; nucleic acid hybridization; nucleic acid probes; nucleic acid sequence; radiotracer; restriction mapping; scanning electron microscopy

Our major aim is to understand the changes in nuclei of aneuploid CNS neoplasms. Isolation and characterization of subsets of repeated sequences in mouse and human DNA will be continued, with emphasis on chromosomal structure (studied by in situ hybridization) and DNA analysis. Ultra-structural studies of the nucleolus organizing regions in interphase nuclei will be correlated with quantitative analysis of DNA restriction profiles from normal and neuroectodermal tumor cells using defined ribosomal DNA probes. Methods to analyze ribosomal and other DNA sequence subsets of interest, with respect to their sequence, distribution, and three-dimensional arrangement in chromosomes and interphase nuclei, are being pursued. Recent progress with biotin-labeled DNA probes has allowed high-resolution (EM) studies of specific DNA sequences in preparations of interphase and metaphase chromosomes. These studies indicate specific three-dimensional arrangements of selected DNA sequences in differentiated CNS cell types and significant alterations in CNS tumor cells. (I)

我们的主要目标是了解非整倍体中枢神经系统细胞核的变化 肿瘤。 重复序列子集的分离和表征 将继续对小鼠和人类 DNA 进行研究，重点是染色体 结构（通过原位杂交研究）和 DNA 分析。 间期核仁组织区的超微结构研究 细胞核将与 DNA 限制性定量分析相关 使用定义的正常和神经外胚层肿瘤细胞的概况 核糖体 DNA 探针。 分析核糖体和其他 DNA 序列的方法 感兴趣的子集，关于它们的顺序、分布和 染色体和间期核的三维排列是 被追击。 生物素标记 DNA 探针的最新进展使得 制剂中特定 DNA 序列的高分辨率 (EM) 研究 间期和中期染色体。 这些研究表明具体 不同DNA序列的三维排列 中枢神经系统细胞类型和中枢神经系统肿瘤细胞的显着变化。 （我）