RNA STUDIES AND THE CREUTZFELDT-JACOB DISEASE AGENT

RNA 研究和克雅氏病病原体

• 批准号: 6112583
• 负责人: laura MANUELIDIS
• 金额: $ 17.32万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6112583
• 关键词: aging; brain; centrifugation; complementary DNA; disease /disorder model; electron microscopy; gel electrophoresis; gene expression; genetic manipulation; hamsters; high performance liquid chromatography; model design /development; molecular cloning; molecular pathology; nervous system infection; nucleic acid hybridization; nucleic acid sequence; pathologic process; phosphorus; prions; radionuclides; radiotracer; spongiform encephalopathy; virus RNA; virus classification; virus protein

The ultimate aim of this project is to define CJD agent specific (non-host) molecules, i.e. proteins and nucleic acids. It is obvious that definition of these elements will be invaluable for the ultimate understanding of these agents and the dementias they induce. The identification of truly agent specific molecules will also permit reliable diagnostic tests for human dementias. The project is split into two parts. The first deals with a definitive evaluation of the size and density of the infectious agent and will be useful for further systematic purification of agent, and detection of agent protein(s). The second part concentrates on detection, amplification and sequencing of specific RNAs (cDNA clones) that may belong to the infectious agent.

该项目的最终目标是定义 CJD 代理具体 （非宿主）分子，即蛋白质和核酸。 这是 显然，这些元素的定义对于 对这些因素及其痴呆症的最终了解 诱导。 真正的药物特异性分子的鉴定将 还可以对人类痴呆症进行可靠的诊断测试。 该项目分为两部分。 第一个涉及一个 传染性大小和密度的明确评估 剂，将有助于进一步系统纯化 剂，以及剂蛋白的检测。 第二部分 专注于特定的检测、扩增和测序 可能属于传染原的 RNA（cDNA 克隆）。