RNA STUDIES AND THE CREUTZFELDT-JACOB DISEASE AGENT

RNA 研究和克雅氏病病原体

• 批准号: 6112583
• 负责人: laura MANUELIDIS
• 金额: $ 17.32万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6112583
• 关键词: aging; brain; centrifugation; complementary DNA; disease /disorder model; electron microscopy; gel electrophoresis; gene expression; genetic manipulation; hamsters; high performance liquid chromatography; model design /development; molecular cloning; molecular pathology; nervous system infection; nucleic acid hybridization; nucleic acid sequence; pathologic process; phosphorus; prions; radionuclides; radiotracer; spongiform encephalopathy; virus RNA; virus classification; virus protein

The ultimate aim of this project is to define CJD agent specific (non-host) molecules, i.e. proteins and nucleic acids. It is obvious that definition of these elements will be invaluable for the ultimate understanding of these agents and the dementias they induce. The identification of truly agent specific molecules will also permit reliable diagnostic tests for human dementias. The project is split into two parts. The first deals with a definitive evaluation of the size and density of the infectious agent and will be useful for further systematic purification of agent, and detection of agent protein(s). The second part concentrates on detection, amplification and sequencing of specific RNAs (cDNA clones) that may belong to the infectious agent.

该项目的最终目的是定义特定于CJD代理 （非宿主）分子，即蛋白质和核酸。 这是 显然，这些元素的定义对于 对这些代理商和痴呆症的最终理解 诱导。 真正特异性分子的识别将 还允许对人痴呆的可靠诊断测试。 该项目分为两个部分。 第一个交易 对感染性的大小和密度的确切评估 代理商将有助于进一步的系统净化 剂和剂蛋白的检测。 第二部分 集中于检测，扩增和测序特定 RNA（cDNA克隆）可能属于感染剂。