Regulation of plant phospholipid biosynthesis

植物磷脂生物合成的调控

基本信息

批准号：
BB/G009724/1
负责人：
Peter Eastmond
金额：
$ 56.48万
依托单位：
University of Warwick
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2009
资助国家：
英国
起止时间：
2009 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FG009724%2F1
关键词：
Regulation plant phospholipid biosynthesis

项目摘要

Phospholipids are essential for the construction of eukaryotic cell membranes, which play a fundamental role in compartmentalising the biochemistry of life. The quantity and composition of phospholipids are tightly regulated during growth and development, and in response to environmental change, so that membranes always maintain their structure and function. Research in mammals and yeast (Saccharomyces cerevisiae) has uncovered elegant metabolite signaling mechanisms, both transcriptional and post-translational, that allow the cell to sense changes in key lipid intermediates and adjust phospholipid synthesis (and turnover) accordingly. Analogous mechanisms are also likely to exist in plants but surprisingly they have not been elucidated. Because phospholipids are essential, genetic analysis of their regulation through loss-of-function is problematic. However, we have recently isolated an Arabidopsis thaliana double mutant in two phosphatidate phosphatases (pah1 pah2) that produces approximately twice as much phospholipid in its leaves as wild type plants. To our knowledge this is the first plant mutant to over-produce phospholipids and the gain-of-function phenotype provides a unique tool. The objective of this grant proposal is to use the pah1 pah2 mutant (and corresponding genes) to discover how phospholipid biosynthesis is regulated in Arabidopsis and to investigate how it is coordinated with cell cycle progression, which requires membrane biogenesis. This discovery will be of fundamental scientific interest, particularly as there is already evidence to show that many key elements of the regulatory mechanism(s) in plants must differ from those described in mammals or yeast.

磷脂对于建造真核细胞膜至关重要，这在分隔生命的生物化学中起着基本作用。磷脂的数量和组成在生长和发育过程中受到严格调节，并响应环境变化，因此膜始终保持其结构和功能。在哺乳动物和酵母（酿酒酵母）中进行的研究发现了转录和翻译后的优雅代谢物信号传导机制，使细胞可以相应地感知脂质中间体的关键脂质中间体的变化，并相应地调整磷脂合成（和流失）。类似机制也可能存在于植物中，但令人惊讶的是尚未阐明它们。因为磷脂是必不可少的，所以通过功能丧失对其调节的遗传分析是有问题的。但是，我们最近在两个磷酸化磷酸酶（PAH1 PAH2）中分离了拟南芥双重突变体，该磷酸化酶（PAH1 PAH2）在其叶片中产生的磷脂是野生型植物的两倍。据我们所知，这是第一个过度生产磷脂的植物突变体，功能获得的表型提供了独特的工具。该赠款提案的目的是使用PAH1 PAH2突变体（和相应的基因）来发现如何在拟南芥中调节磷脂生物合成，并研究其与细胞周期进程的协调，这需要膜生物发生。这一发现将具有基本的科学兴趣，特别是因为已经有证据表明植物中监管机制的许多关键要素必须与哺乳动物或酵母中描述的植物不同。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The Arabidopsis thaliana RNA editing factor SLO2, which affects the mitochondrial electron transport chain, participates in multiple stress and hormone responses.

DOI：
10.1093/mp/sst102
发表时间：
2014-02
期刊：
Molecular plant
影响因子：
27.5
作者：
Qiang Zhu;Jasper Dugardeyn;Chunyi Zhang;P. Mühlenbock;P. Eastmond;R. Valcke;B. De Coninck;Sevgi Oden;Michael Karampelias;B. Cammue;E. Prinsen;D. Van Der Straeten
通讯作者：
Qiang Zhu;Jasper Dugardeyn;Chunyi Zhang;P. Mühlenbock;P. Eastmond;R. Valcke;B. De Coninck;Sevgi Oden;Michael Karampelias;B. Cammue;E. Prinsen;D. Van Der Straeten

Transmembrane domain length is responsible for the ability of a plant reticulon to shape endoplasmic reticulum tubules in vivo.

跨膜结构域长度决定植物网网在体内塑造内质网小管的能力。

DOI：
10.1111/j.1365-313x.2010.04337.x
发表时间：
2010
期刊：
for cell and molecular biology
影响因子：
0
作者：
Tolley N
通讯作者：
Tolley N

Arabidopsis uses two gluconeogenic gateways for organic acids to fuel seedling establishment.

DOI：
10.1038/ncomms7659
发表时间：
2015-04-10
期刊：
NATURE COMMUNICATIONS
影响因子：
16.6
作者：
Eastmond, Peter J.;Astley, Holly M.;Parsley, Kate;Aubry, Sylvain;Williams, Ben P.;Menard, Guillaume N.;Craddock, Christian P.;Nunes-Nesi, Adriano;Fernie, Alisdair R.;Hibberd, Julian M.
通讯作者：
Hibberd, Julian M.

Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity.