NEUROTRANSMITTER RECEPTORS

神经递质受体

• 批准号: 3075373
• 负责人: SOLOMON H. SNYDER
• 金额: $ 6.03万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-07-01 至 1990-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3075373
• 关键词: adenosine; amine oxidase (flavin); antibody formation; autoradiography; calcium channel blockers; captopril; carboxypeptidase; chemical binding; chemical structure function; human tissue; molecular site; monoclonal antibody; neural transmission; neurotoxins; neurotransmitter metabolism; peptidyl dipeptidase A; phorbols; protein kinase C; psychotomimetic drug; psychotropic drugs; synapses; tissue /cell culture

My aims for research during the coming five years include a number of different projects. One has to do with the characterization of enkephalin convertase, a carboxypeptidase which appears to be associated with the biosynthesis of enkephalin. We will try to measure the turnover of the enzyme and evaluate in detail its localization. Another project relates to angiotensin converting enzyme which can be labeled with the inhibitor H-3-captopril. We propose to attempt to identify what seems to be a novel neuropeptide which is the endoyenous substrate for this enzyme. We propose to localize endogenous adenosine and ascertain its possible neutrotransmitter role. We will continue studies of calcium antagonist receptors and their relationship to calcium flux in neurons. We will characterize biogenic amine uptake sites with a variety of ligands. We propose studies to further elucidate the function of sigma receptors which are associated with the psychotomimetic effects of opiates. We propose detailed investigations of phorbol ester receptors to clarify their relationship to protein kinase C in neuronal function. We will also evaluate the relationship of the receptor interactions fo neutoxin MPTP with monamine oxiuase.

我未来五年的研究目标包括一些 不同的项目。 一与脑啡肽的表征有关 转化酶，一种羧肽酶，似乎与 脑啡肽的生物合成。 我们将尝试衡量营业额 酶并详细评估其定位。 另一个项目涉及 可以用抑制剂标记的血管紧张素转换酶 H-3-卡托普利。 我们建议尝试识别什么似乎是小说 神经肽，它是该酶的内质底物。 我们建议 定位内源性腺苷并确定其可能性 中性递质的作用。 我们将继续钙拮抗剂的研究 受体及其与神经元钙通量的关系。 我们将 用各种配体表征生物胺吸收位点。 我们 提出研究进一步阐明西格玛受体的功能 与阿片类药物的拟精神病作用有关。 我们建议 详细研究佛波酯受体以阐明其 神经元功能中蛋白激酶 C 的关系。 我们还将 评估中毒素 MPTP 受体相互作用的关系 与单胺氧化酶。