LOOKING FOR RNA CARGOS REGULATED BY FMRP

寻找受 FMRP 监管的 RNA 货物

• 批准号: 7358006
• 负责人: WILLIAM T GREENOUGH
• 金额: $ 1.26万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-29 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358006
• 关键词: RNA; RNA binding protein; amine oxidase (flavin); fragile X syndromes; genetically modified animals; human; intracellular transport; laboratory mouse; mental retardation; neurons; proteins

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. FMRP is the protein mutated from fragile X syndrome, which is a common mental retardation in human. FMRP is also a RNA binding protein and may regulate RNA transport in neurons and its translation. We identified several RNAs associated with FMRP by APRA-microarray. One of them is R1, which is a transcription regulator of Monoamine oxidase-A (MAO-A). Now we are tried to detect the MAO-A activity in wild type and FMRP knockout mice. And the MAO substrate we are using is fluorescent protein- Kynuramine. The technique in LFD is very important for us to get the results.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。 FMRP是从脆弱X综合征突变的蛋白质，这是人类的常见智力低下。 FMRP也是RNA结合蛋白，可能调节神经元中的RNA转运及其翻译。我们确定了与APRA-Microarray相关的几个与FMRP相关的RNA。其中之一是R1，它是单胺氧化酶A（MAO-A）的转录调节剂。现在，我们试图检测野生型和FMRP敲除小鼠中的MAO-A活性。我们正在使用的MAO底物是荧光蛋白基瘤。 LFD中的技术对我们来说非常重要。