PROTECTIVE EPITOPES IN TICK IMMUNITY

蜱免疫中的保护性表位

• 批准号: 2887398
• 负责人: FRED S KANTOR
• 金额: $ 29.37万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2887398
• 关键词: Borrelia; Ehrlichia; Ixodes; Lyme disease; antigen antibody reaction; arthropod borne communicable disease; bacterial antigens; bactericidal immunity; borreliosis; communicable disease transmission; ehrlichiosis; genetic library; guinea pigs; high performance liquid chromatography; host organism interaction; immunoaffinity chromatography; immunofluorescence technique; laboratory mouse; molecular cloning; nucleic acid sequence; passive immunization; polymerase chain reaction; protein purification; salivary glands; surface antigens; western blottings

The proposed project is to identify the molecular basis of the antigens involved in tick immunity and study its effects upon the transmission of Lyme borreliosis and Ehrlichiosis. Specifically a model of tick protective immunity has been established in guinea pigs. Antibodies already shown to be capable of passively protecting recipients will be purified using chemical and immunoabsorbent methods. Tick salivary antigens will be obtained for Ixodes scapularis; these antigens will be purified by chemical and immunological means using the purified protective antibodies described above. Such antigens will be used to actively produce tick immunity in the model. With purified antibodies obtained from tick-sensitized animals, cDNA libraries already prepared from salivary glands will be screened. Such libraries, prepared from stimulated tick salivary glands, will be used to clone genes encoding protective antigens. Several putative clones have already been identified. Recombinant antigens will be expressed, purified, and characterized before use as immunogens to immunize rice and guinea pigs to determine their potential for induction of anti-tick immunity. Using immune guinea pigs, the effect of anti-tick immunity on the ability of ticks infected with either Borrelia burgdorferi or Ehrlichia canis to transmit disease to tick-immune hosts will be determined. The kinetics of transmission from infecting tick to guinea pig host will be the basis of studies on the mechanism of how anti-tick immunity affects pathogen transmission.

拟议的项目是确定抗原的分子基础 参与tick免疫并研究其对传播的影响 莱姆毛毛虫病和ehrlichiisois。 特别是在 豚鼠。抗体已经证明能够被动 保护接受者将使用化学和免疫吸光剂纯化 方法。将获得ixodes capularis的滴答唾液抗原； 这些抗原将通过化学和免疫学手段纯化 上述纯化的保护性抗体。这样的抗原将是 用于在模型中积极产生壁虱免疫。 纯化的抗体从tick敏化的动物中获得 将筛选已经从唾液腺准备的库。这样的 由刺激滴答唾液腺制备的图书馆将用于 编码保护性抗原的克隆基因。一些推定的克隆有 已经确定了。 重组抗原将在之前表达，纯化和表征 用作免疫原以免疫水稻和豚鼠来确定其 诱导抗挑剔免疫力的潜力。 使用免疫豚鼠，反挑战免疫对能力的影响 感染了伯氏毛burgdorferi或ehrlichia canis的壁虱 将确定向tick虫免疫宿主的疾病。动力学 从感染壁虱传播到豚鼠宿主将是 研究抗小便免疫如何影响病原体的机制 传播。