PROTECTIVE MECHANISMS IN THE INTERFACE BETWEEN SPIROCHETE, TICK AND HOST

螺旋体、蜱虫和宿主之间界面的保护机制

• 批准号: 6268036
• 负责人: FRED S KANTOR
• 金额: $ 16.52万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6268036
• 关键词: Borrelia; Ixodes; Lyme disease; SDS polyacrylamide gel electrophoresis; active immunization; antigens; bactericidal immunity; communicable disease transmission; disease /disorder model; disease vectors; genetic library; guinea pigs; host organism interaction; humoral immunity; immunity; immunoglobulin G; laboratory mouse; laboratory rabbit; molecular cloning; monoclonal antibody; passive immunization; protein purification; salivary glands; surface antigens; tissue /cell culture; western blottings

Acquired resistance to ticks was reported in the late l93Os. These findings have been confirmed and extended to guinea pigs and rabbits using several species of ticks by several investigators including ourselves. The proposed project is to identify the molecular basis of the antigens involved in tick immunity and study its effect on the antigens of the B. burgdorferi carried by the ticks and upon the transmission of Lyme borreliosis. Specifically models of tick protective immunity will be established in guinea pigs and in rabbits. Antibodies shown to be capable of passively protecting recipients will be purified using chemical and immunoabsorbent methods. Antigen specificity of lymphocytes from tick protected animals and from clones will be characterized. Tick Salivary and midgut antigens will be obtained from the appropriate organs of Ixodes scapularis and from supernatants and homogenates of tissue cultures; these antigens will be purified by chemical and immunological means. Such antigens will be used to produce tick immunity in the models chosen. With polyclonal and monoclonal antibodies obtained from tick-or purified antigen-sensitized animals DNA libraries will be screened. Such libraries, prepared from stimulated tick salivary glands and midguts, will be used to clone genes encoding protective antigens. Using immune guinea pigs and mice the effect of anti-tick immunity on the surface antigens of tick-borne Borrelia burgdorferi will be studied and the ability of infected ticks to transmit disease to tick-immune host determined. The potential synergistic effects of dual anti-tick and anti- Osp A immunity on the ability of infected ticks to transmit Lyme borreliosis will be investigated in the mouse model.

在L93OS后期，据报道了对tick的抗药性。 这些 发现结果已被确认并扩展到豚鼠和兔子 包括我们自己在内的几个调查人员的几种tick虫。 拟议的项目是确定抗原的分子基础 参与tick免疫力并研究其对B的抗原的影响。 滴答tick和莱姆传播后携带的汉堡 毛毛病。 特定的壁虱保护豁免模型将在 豚鼠和兔子。证明能够被动的抗体 保护接受者将使用化学和免疫吸光剂纯化 方法。受保护动物的淋巴细胞的抗原特异性 并将从克隆中进行特征。 滴答唾液和中肠抗原将从适当的 ixodes的器官肩cap骨以及上清液和匀浆 组织培养；这些抗原将通过化学和 免疫学手段。 这种抗原将用于产生壁虱免疫力 在选择的模型中。 从滴答或纯化的多克隆和单克隆抗体中 将筛选抗原敏感的动物DNA文库。 这样的 由刺激的滴答唾液腺和中肠制备的图书馆将 用于克隆编码保护抗原的基因。 使用免疫豚鼠和小鼠抗小便免疫对 将研究tick传播的Borrelia burgdorferi的表面抗原 感染壁虱将疾病传播到tick tick-ammune宿主的能力 决定。 双反式和反抗的潜在协同作用 OSP对传播莱姆的感染能力的免疫力 小鼠模型将研究毛毛虫病。