SPECTROSCOPIC SPECIATION OF SULFUR IN WHOLE CELLS

全细胞中硫的光谱形态

• 批准号: 6019419
• 负责人: GRAHAM N GEORGE
• 金额: $ 9.75万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6019419
• 关键词: X ray spectrometry; biomedical equipment development; cryoscience; electronic spectra; method development; radiochemistry; sulfur

DESCRIPTION: (applicant's abstract) Biochemistry revolves around the chemistry of carbon, but many other elements play vital roles. Sulfur is such an element, with roles in structure, catalysis and metabolism in all organisms. Specifically in humans, homocysteinemia, together with low S-adenosyl methionine levels, has been implicated in coronary artery disease and in atherosclerotic disease. Our goal is to develop sulfur K-edge X-ray absorption near-edge spectroscopy so that it can be used to monitor and to improve our understanding of sulfur metabolism in living systems. There are currently no effective spectroscopic probes of sulfur in biological systems. X-ray absorption spectroscopy will detect all chemical forms of sulfur in a sample, in solid, in aqueous solution or in any other form, and different forms of sulfur display strikingly different spectra. No pre-treatment is needed and the technique is at least potentially non-destructive. The ultimate potential of the work we propose herein is to provide an in vivo probe of metabolic status. To this end, we propose the following specific aims for this initial study: 1. Generate a library of sulfur spectra of a range of biologically significant compounds under a range of physiologically relevant conditions. 2. Develop statistically rigorous curve-fitting routines for evaluation of complex mixtures, and quantitatively evaluate particle size and concentration effects. 3. Evaluate the potential problems from radiation damage, including the requirement for cryo-protection of the sample. 4. Validate our approach by initial studies of simple biological systems where sulfur plays very major roles in metabolism. 5. Apply the developed techniques to more challenging eukaryotic systems. This latter aim may not be achieved in the initial period of the grant.

描述：（申请人的摘要）生物化学围绕 碳化学，但许多其他元素都起着至关重要的作用。 硫是 这样的元素，在结构，催化和代谢中的作用 有机体。 特别是在人类中，同型囊性血症 S-腺苷蛋氨酸水平已与冠状动脉疾病有关 以及动脉粥样硬化疾病。 我们的目标是开发硫K-Edge X射线 吸收近边光谱学，因此可以用于监测和进行 提高我们对生活系统中硫代谢的理解。 有 目前尚无生物系统中硫的有效光谱探针。 X射线吸收光谱将检测A中的所有化学形式的硫 样品，固体，水溶液或任何其他形式，不同 硫的形式显示出明显不同的光谱。 没有预处理 所需的技术至少是潜在的无损。 这 我们在此提出的工作的最终潜力是提供体内 代谢状态的探测。 为此，我们提出了以下最初研究的具体目的： 1。生成一系列生物学的硫光谱库 在一系列生理相关条件下的重要化合物。 2。开发统计上严格的曲线拟合程序以评估 复杂的混合物，并定量评估粒度和 浓度效应。 3。评估辐射的潜在问题 损坏，包括样品冷冻保护的要求。 4。 通过对简单生物系统的初步研究来验证我们的方法，其中 硫在代谢中起着非常重要的作用。 5。应用开发的 更具挑战性的真核系统的技术。 后一个目标可能不会 可以在赠款的初期实现。