ROBOTIC CRYSTAL MOUNTING & ALIGNMENT SYSTEMS

机器人晶体安装

• 批准号: 6834578
• 负责人: Thomas N Earnest
• 金额: $ 41.53万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6834578
• 关键词: X ray crystallography; bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; computer program /software; cryoscience; crystallization; data collection methodology /evaluation; fiber optics; fluorescence spectrometry; high throughput technology; imaging /visualization /scanning; micromanipulator; protein structure; proteomics; robotics; structural biology

This proposal is to develop and test an integrated assembly of robotics subsystems dedicated to high-throughput protein crystallography at a synchrotron facility. The long-term goal is the implementation of an automated environment for the efficient manipulation of large numbers of single crystal protein samples for x-ray diffraction intensity collection at synchrotron-based biological crystallography facilities. Specific subtasks include the design and implementation of manual, semi- automated, and automated remote control systems for sample manipulation on the goniometer and in x-ray beam, development of algorithms for automated centering of target features, design and testing of a subsystem to load and unload conventional cryogenically-frozen protein crystal samples, development of protocols for screening large numbers of crystals for data collection, development and testing of new sample holders for efficient crystal harvesting, storage, manipulation, and data collection, and finally, adaptation of the hardware to the alignment of microcrystals. Although there are currently more than a dozen synchrotron beamlines in the U.S. dedicated to protein crystallography, the throughput is limited by the speed of manual manipulation (loading, centering, and unloading). At the Macromolecular Crystallography Facility at Advanced Light Source, one current beamline, two new beamlines under construction, and several new beamlines being designed, will benefit from the use of automated stages -initially a simple x,y,z movement with motors under manual control - for centering protein crystals. The proposed integrated approach for automated manipulation of protein crystals will be more efficient (in terms of time and synchrotron floor space), and will substantially increase throughput and reliably in handling samples. This proposal is in direct response to the recommendations of the NIH and DOE in designing automated systems for use at synchrotron facilities and in developing technologies necessary for advancing high-throughput structural genomics initiatives.

该建议是开发和测试专门针对同步器设施上高通量蛋白质晶体学的机器人系统子系统的集成组装。长期目标是实施自动化环境，以有效操纵基于同步加速器的生物学晶体学设施的大量单晶蛋白样品来进行X射线衍射强度收集。具体的子任务包括手动，半自动化和自动化遥控系统的设计和实施，用于操纵性能仪和X射线光束，开发目标特征的算法，用于自动化目标特征，设计和测试子系统以负载和未加载的量子和未载荷的新型蛋白质蛋白质水晶的开发，以实现量的新样品，以实现群体的开发，以筛选群体的开发，并开发了群体的开发。为了有效的晶体收获，存储，操纵和数据收集，最后是将硬件适应微晶对齐的。尽管目前在美国有十几个同步束线，专用于蛋白质晶体学，但吞吐量受到手动操纵速度（负载，居中和卸载）的限制。在高级光源的大分子晶体学设施上，一个电流梁线，两个新的光束线以及正在设计的几种新梁，将受益于使用自动阶段的使用 - 从小手动控制自动X，y，z运动，该动力在手动控制下 - 用于中心蛋白质晶体。提出的用于自动操纵蛋白质晶体的综合方法将更有效（在时间和同步底空间方面），并将大大增加吞吐量并可靠地处理样品。该提案直接回应NIH和DOE在设计自动化系统中的建议，以在同步加速器设施中使用以及开发用于推进高通量结构基因组学计划所必需的技术。