Beamline Automation for Structure Determination

用于结构测定的光束线自动化

• 批准号: 7037118
• 负责人: Thomas N Earnest
• 金额: $ 52.13万
• 依托单位: UNIVERSITY OF CALIF-LAWRENC BERKELEY LAB
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7037118
• 关键词: X ray crystallography; biomedical automation; biotechnology; computer program /software; computer system hardware; fluorescence spectrometry; high throughput technology; micromanipulator; protein structure; structural biology; synchrotrons; technology /technique development

DESCRIPTION (provided by applicant): This proposal is to request funding for a competing renewal of GM62648 to further develop tools and automation for x-ray crystallography of biological molecules. These tools have wide benefit to the structural biology community as they provide improved and optimized approaches to x-ray data collection and processing, as well as improvements and new methods for researchers to interact with the complex instrumentation at synchrotron crystallography beamlines. The synchrotron beamlines provide the majority of x-ray data for structural biology, and enable the pursuit of ever more challenging structural projects such as the ribosome, RNA polymerase complexes, and integral membrane proteins. The development of highly-automated technologies and methods is critical to the productivity of the scientific community and is necessary to increase the high- throughput capabilities for structural genomics and drug discovery projects. Over the past four years, we have designed, implemented, and tested an automated crystal mounting and alignment system, integrated it into the beamline control system in sector 5 at the Advanced Light Source, extensively tested and improved it in response to the tests and user feedback, started an effort with the group of Paul Adams (LBNL) to more fully automate data collection and processing, and begun the collaborative propagation of the system to other synchrotron beamlines. For this renewal, we propose to focus on the following goals: 1) Upgrade of the design of the automated sample- handling system - finalizing hardware of first generation system and improvements in the gripper assembly, and the design, implement, and testing second-generation system with cartesian geometry; 2) Improve and more fully automate crystal recognition and alignment; 3) Improve and more fully automate the sample-tracking subsystem; 4) Develop a user-directed remote measurement and information management capability. These technologies are generalizable to the other sites and to a broad range of user needs.

描述（由申请人提供）：该提案是要求为竞争续签GM62648的竞争提供资金，以进一步开发生物分子X射线晶体学的工具和自动化。这些工具为结构生物学社区提供了广泛的好处，因为它们为X射线数据收集和处理提供了改进和优化的方法，以及研究人员与同步晶体晶体学光束线上复杂仪器互动的改进和新方法。同步束线条提供了结构生物学的大部分X射线数据，并能够追求越来越具有挑战性的结构项目，例如核糖体，RNA聚合酶复合物和整体膜蛋白。高度自动化的技术和方法的发展对于科学界的生产力至关重要，对于提高结构性基因组学和药物发现项目的高吞吐量能力是必不可少的。 Over the past four years, we have designed, implemented, and tested an automated crystal mounting and alignment system, integrated it into the beamline control system in sector 5 at the Advanced Light Source, extensively tested and improved it in response to the tests and user feedback, started an effort with the group of Paul Adams (LBNL) to more fully automate data collection and processing, and begun the collaborative propagation of the system to other synchrotron梁线。对于此续约，我们建议专注于以下目标：1）升级自动化样品处理系统的设计 - 最终确定第一代系统的硬件和抓地力组装的改进，以及使用笛卡尔几何形状的设计，实施和测试第二代系统； 2）改进并更完全自动化晶体识别和对齐； 3）改进并更完全自动化样品跟踪子系统； 4）开发以用户指导的远程测量和信息管理功能。这些技术可推广到其他站点以及广泛的用户需求。