60 HZ MAGNETIC FIELD EFFECTS ON CHOLINERGIC SYSTEM

60 HZ 磁场对胆碱能系统的影响

基本信息

批准号：
2518657
负责人：
HENRY C LAI
金额：
$ 19.4万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
1994
资助国家：
美国
起止时间：
1994-09-28 至 1999-02-28
项目状态：
已结题

项目摘要

We propose experiments to study the effects of extremely low-frequency (ELF) magnetic field exposure on neurological functions and behavior in the rat. From the data of our preliminary experiments, we hypothesize that acute exposure to ELF magnetic fields activates endogenous opioids, which in turn causes a decrease in cholinergic activity in the brain. These changes in central cholinergic activity lead to a deficit in learning and memory functions. We intend to further investigate and characterize this chain of events through the following experiments: (1) A study to investigate the effects of acute exposure to magnetic fields of different combinations of intensity and duration, and of different frequencies on sodium-dependent high-affinity choline uptake (HACU), an index of cholinergic activity, in different regions of the rat brain. (2) A study to investigate the effects of repeated magnetic field exposure on HACU and muscarinic cholinergic receptors in different regions of the brain. (3) A study to investigate the involvement of opioid receptor subtypes (mu, delta, and kappa) on the effect of magnetic fields on cholinergic systems; and (4) A study to investigate the effect of magnetic field exposure on performance in the radial-arm maze, a behavioral task involving spatial memory functions. Since it is well known that radial-arm maze performance depends on central cholinergic activity, behavioral deficits will be correlated with the changes in cholinergic activity in the brain. It is hoped that the data from these proposed studies will identify the exposure conditions that may trigger deleterious effects, reveal the neural mechanisms affected by ELF magnetic fields, and help in the risk assessment and setting of magnetic field-exposure guidelines in the public and occupational environments.

我们提出了研究极低频率的效果的实验（精灵）在神经功能和行为上暴露于磁场大鼠。根据我们的初步实验的数据，我们假设急性暴露于小精灵磁场会激活内源性阿片类药物，反过来，这会导致大脑中胆碱能活性的降低。中央胆碱能活性的这些变化导致学习和记忆功能。我们打算进一步调查和通过以下实验表征这一事件链：（1）研究急性暴露于磁场的影响的研究强度和持续时间的不同组合以及不同的组合依赖钠的高亲和力胆碱吸收（HACU）的频率大鼠大脑不同区域的胆碱能活性指数。（2）研究重复磁场暴露的影响的研究在HACU和毒蕈碱胆碱能受体上脑。（3）研究阿片受体参与的研究亚型（MU，Delta和Kappa）对磁场对胆碱能系统；（4）研究研究的研究 radial臂迷宫中性能的磁场暴露涉及空间内存功能的行为任务。因为很好知道径向臂迷宫的性能取决于中央胆碱能活动，行为缺陷将与变化相关大脑中的胆碱能活性。希望来自这些的数据拟议的研究将确定可能触发的暴露条件有害效果，揭示了受精灵影响的神经机制磁场，并有助于磁性评估和设置公共和职业环境中的现场暴露指南。