60 HZ MAGNETIC FIELD INDUCED DNA DAMAGE

60 HZ 磁场引起的 DNA 损伤

基本信息

批准号：
2634353
负责人：
HENRY C LAI
金额：
$ 7.41万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-01-18 至 1999-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2634353
关键词：
DNA damage DNA repair brain cell free radical oxygen free radical scavengers laboratory rat magnetic field melatonin nutrition related tag pharmacogenetics radiation genetics tocopherols vitamin analog

项目摘要

Recent research in our laboratory shows that rats acutely (2 hrs) exposure to a 60-Hz magnetic field (0.1 - 0.5 mT) have an increase in DNA single and double strand breaks in their brain cells. Experiments are proposed to further investigate the mechanism by which exposure to a 60 Hz magnetic field causes DNA damages in brain cells of the rat. Specifically, the role played by free radicals will be investigated, since our preliminary research also shows that treatment of rats before exposure with free radical scavengers (melatonin and a spin trap compound) can block the DNA damaging effect of magnetic fields. Free radicals have recently been implicated in the biological effects of extremely low frequency (ELF) magnetic fields. Experiments are designed to investigate the involvement of free radicals in magnetic field- induced DNA strand breaks in cells. This proposed research has the following specific aims: (1) To further confirm the blocking effect of melatonin on magnetic field-induced DNA single and double strand breaks in brain cells of rats, a (drug) dose-response study will be carried out. (2) To provide further support for the hypothesis that free radicals are involved in the effect of magnetic fields, animals will be treated with different spin-trap compounds and the vitamin E analog trolox to investigate whether they can block the effects of magnetic fields on DNA strand breaks in rat brain cells. Spin-trap compounds and trolox are efficient free radical scavengers. This is an important new direction in ELF electromagnetic field bioeffect research because DNA damage is closely related to human health risk. Particularly, DNA damage in brain cells could affect neurological functions and also possibly lead to carcinogenesis and neurodegenerative diseases. It is hoped that data from this proposed research will help in identifying possible causal connections of exposure to power frequency magnetic fields and biological effects and also in the hazard assessment of power frequency electromagnetic fields.

我们实验室的最新研究表明，大鼠急性（2小时）暴露于60 Hz磁场（0.1-0.5 mt）的增加 DNA单链和双链在其脑细胞中断裂。实验建议进一步研究暴露于 60 Hz磁场会导致大鼠脑细胞的DNA损伤。具体而言，将研究自由基的角色，由于我们的初步研究也表明，治疗之前的大鼠自由基清除剂（褪黑激素和自旋陷阱）接触化合物）可以阻止磁场的DNA损伤效应。自由的最近的激进分子与极低的频率（ELF）磁场。设计实验研究自由基参与磁场诱导的DNA链在细胞中断裂。这项拟议的研究具有遵循特定目的：（1）进一步确认褪黑激素对磁的阻塞效应野外诱导的DNA单链和双链断裂的脑细胞中断裂大鼠将进行一项（药物）剂量反应研究。（2）为自由基是的假设提供了进一步的支持参与磁场的影响，将用动物处理不同的自旋陷阱化合物和维生素E模拟trolox to 研究它们是否可以阻止磁场对DNA的影响大鼠脑细胞中的链断裂。自旋陷阱化合物和Trolox是有效的自由基清除仪。这是精灵电磁场的重要新方向生物效应研究是因为DNA损伤与人类健康密切相关风险。特别是，脑细胞中的DNA损伤可能会影响神经系统功能，还可能导致致癌和神经退行性疾病。希望这项拟议的研究的数据将有助于在确定暴露于电力的可能因果关系时频率磁场和生物学效应以及危险中电力频率电磁场的评估。