SSPF A LATE FORSPORE GENE PRODUCT

SSPF 晚期福孢子基因产品

• 批准号: 2166015
• 负责人: CHARLES A LOSHON
• 金额: $ 3.53万
• 依托单位: UNIVERSITY OF MARYLAND EASTERN SHORE
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2166015
• 关键词: Bacillus subtilis; DNA binding protein; Escherichia coli; SDS polyacrylamide gel electrophoresis; bacterial genetics; cell population study; developmental genetics; gene expression; genetic manipulation; genetic strain; longevity; microorganism reproduction; molecular cloning; protein sequence; protein structure function; radionuclides; southern blotting; spores

The long-term objective of the proposed research is to study developmental events which occur during Bacillus subtilis sporulation. The immediate objective is to investigate sspF, a forespore specific gene which specifies a stable, abundant but poorly translated mRNA. The deduced amino acid sequence of sspF is very highly conserved and the sspF gene product may have essential functions related to spore resistance and longevity. The specific aims are to (a) identify the sspF gene product, (b) isolate and characterize it and (c) determine its function. The research design and methods involve the construction of B. Subtilis strains in which the sspF gene is either deleted or overexpressed in order to determine the effect on spore formation, germination and essential properties. The proposed research should provide additional information on the molecular events leading to spore formation, resistance and longevity. Since these events are common to all sporeformers, this information is applicable to the medically important, pathogenic members of the Bacillus and Clostridium genera.

拟议研究的长期目标是研究发育 枯草芽孢杆菌孢子形成过程中发生的事件。 即时的 目的是研究 sspF，一种前孢子特异性基因， 指定一个稳定、丰富但翻译较差的 mRNA。 推导出来的 sspF 的氨基酸序列非常保守，并且 sspF 基因 产品可能具有与孢子抗性相关的基本功能 长寿。 具体目标是 (a) 鉴定 sspF 基因产物， (b) 分离并表征它，(c) 确定其功能。 这 研究设计和方法涉及枯草芽孢杆菌的构建 sspF 基因按顺序被删除或过表达的菌株 确定对孢子形成、萌发和必需物质的影响 特性。 拟议的研究应提供额外的信息 导致孢子形成、抵抗力和 长寿。 由于这些事件对于所有孢子形成者来说都是常见的，因此 信息适用于具有医学重要性的致病成员 芽孢杆菌属和梭菌属。