GENE EXPRESSION DURING DEVELOPMENT OF MYXOBACTERIA

粘细菌发育过程中的基因表达

• 批准号: 2174818
• 负责人: SUMIKO INOUYE
• 金额: $ 23.4万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2174818
• 关键词: Escherichia coli; Myxococcus; bacterial genetics; cell differentiation; cell free system; cell motility; developmental genetics; fusion gene; gene expression; guanine nucleotide binding protein; membrane proteins; membrane structure; microorganism growth; molecular cloning; protein S; protein biosynthesis; protein engineering; protein kinase; protein sequence; protein structure; recombinase; southern blotting

Myxococcus xanthus is an excellent prokaryotic system for studying the regulation of development. M. xanthus shows a developmental cycle which is similar to the eukaryotic slime molds such as Dictyostelium discoideum. However, there are several distinct advantages to studying development in M. xanthus, since it is a simple gram-negative bacterium. We propose to study morphogenesis and the molecular mechanisms of control of gene expression during development using M. xanthus as a model system. We will continue to work on the on-going projects such as characterization of development-specific sigma factors, expression of development-specific proteins, GTP-binding proteins and msDNAs. We will also attempt to explore several new aspects of M. xanthus including protein kinases and membrane proteins associated with cell motility and morphogenesis. In this proposal, we will pursue the following specific projects: (1) Identification and characterization of development-specific sigma factors. Using the rpoD gene of M. xanthus [the gene for the vegetative sigma factor (sigA)] as a probe, we will continue to clone various development-specific sigma factors and to investigate their roles in differentiation. (2) Protein U and S. In contrast to protein S (an early developmental protein), protein U is a typical late-developmental protein. The gene for protein U and S will be carried out to elucidate how they are secreted and assembled on the spore surface. (3) GTP binding proteins and protein kinases. In addition to various GTP-binding proteins, recently we have found that M. xanthus contains a gene coding for a protein with significant sequence similarity to eukaryotic protein kinases. We will not only characterize this gene and its role in M. xanthus but also further explore to find other genes for protein kinases. (4) Characterization of the major inner membrane protein. We have found that the inner membrane fraction of M. xanthus contains a protein of 130 kd unusually in a large amount. We have recently cloned the gene for this protein and will characterize it for its possible roles in cellular motility and morphogenesis. (5) RecA proteins. We found that M. xanthus contains two recA genes. We will characterize these genes in terms of their functions in the life cycle. (6) Expression and roles of retrons-Mx65 and -Mx162. M. xanthus contains two independent retrons producing msDNA-Mx65 and msDNA-Mx162, respectively. We will further explore their roles and explore their roles and expression during the life cycle.

粘粒叶thus是一个出色的核系统，用于研究 调节发展。 Xanthus M.显示一个发育周期 类似于真核粘液胶模具，例如迪斯特尔迪斯特尔。 但是，研究发展的发展有几个不同的优势 M. Xanthus，因为它是一种简单的革兰氏阴性细菌。 我们建议 研究形态发生和基因控制的分子机制 在开发过程中使用Xanthus作为模型系统的发育表达。 我们将 继续从事正在进行的项目，例如 开发特异性的Sigma因子，发育特异性的表达 蛋白质，GTP结合蛋白和MSDNA。 我们还将尝试探索 黄菌的几个新方面，包括蛋白激酶和膜 与细胞运动和形态发生相关的蛋白质。 在此提案中，我们将追求以下特定项目：（1） 开发特异性西格玛因子的识别和表征。 使用Xanthus的RPOD基因[营养Sigma因子的基因 （SIGA）]作为探针，我们将继续克隆各种特定开发的特定于 Sigma因素并研究其在分化中的作用。 （2） 蛋白U和S.与蛋白质S相反（早期发育 蛋白质），蛋白U是一种典型的晚期蛋白质。 基因 将进行蛋白质U和S，以阐明它们的分泌方式，并且 组装在孢子表面。 （3）GTP结合蛋白和蛋白质 激酶。 除了各种GTP结合蛋白外，最近我们都有 发现Xanthus M.含有一个编码具有显着蛋白质的蛋白质的基因 与真核蛋白激酶的序列相似性。 我们不仅会 表征该基因及其在Xanthus M. o. Xanthus中的作用 找到其他用于蛋白激酶的基因。 （4）专业的表征 内膜蛋白。 我们发现的内膜分数 M. Xanthus含有大量蛋白质的蛋白质，含量为130 kd。我们 最近将该蛋白质的基因克隆，并将其表征 它在细胞运动和形态发生中的可能作用。 （5）reca 蛋白质。 我们发现Xanthus M.含有两个RECA基因。 我们将 以这些基因在生命周期中的功能来表征这些基因。 （6）反替补-MX65和-MX162的表达和作用。 M. Xanthus包含 两个独立的回试，分别产生MSDNA-MX65和MSDNA-MX162。 我们将进一步探索他们的角色，并探索他们的角色和表达 在生命周期中。