INHIBITORY SYNAPTIC TRANSMISSION IN THE RETINA

视网膜中的抑制性突触传递

• 批准号: 2164118
• 负责人: CHEN-YU YANG
• 金额: $ 12.18万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-01-01 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2164118
• 关键词: GABA receptor; Golgi apparatus; Urodela; amacrine cells; axon; chemical binding; dendrites; gamma aminobutyrate; ganglion cell; glutamates; horseradish peroxidase; immunocytochemistry; larva; molecular site; morphology; neural inhibition; neural transmission; neuroanatomy; receptor binding; retinal bipolar neuron; synapses; transmission electron microscopy

The long term goal if the proposed research is to understand the inhibitory synaptic transmission in the inner retina of the vertebrates. Emphasis will be on the bipolar cells that contain inhibitory neurotransmitter, GABA (GABA-IR) in the tiger salamander retina. The hypothesis to be addressed is: GABA containing bipolar cells are an origin of sustained inhibitory inputs to the ON and OFF ganglion cells thereby providing the anatomical substrate for push-pull modulation of ganglion cell responses. Morphological and electrophysiological techniques will be used on single cells. The specific aims of this project are: (1) characterized the morphological types of HRP injected and/or Golgi stained bipolars. Single bipolar cells in the superfused retinal slices will be strained either with HRP injection or a Golgi collinear method in the isolated retinas; morphology will be studied with camera lucida and a Eutectic Neuron Tracing system; (2) identify the neurotransmitter content (GABA, glutamate) of different morphological types of labeled bipolar cells. GABA and glutamate content of the stained cells will be determined by post-embed immunofluorescence double labelling method at the light microscope (EM) level. (3) study the synaptic input and output target (amacrine cell and/or ganglion cell, bipolar cell) of GABA-IR bipolar cell at the electron microscope (EM) level. Results will be compared to bipolar cells that contain glutamate; (4) determine the synaptic connectivity of GABA-IR bipolar cells and physiologically identified and HRP injected ON and OFF sustained ganglion cell will be OFF light responses will be recorded intracellularly and the ganglion cell will be subsequently stained with HRP. The GABAergic and glutamatergic synaptic input from bipolar cells to the HRP stained ganglion cell will be studied using a post-embed immunogold method at the EM level. The retinas of urodele amphibians including salamanders, have been used for over 25 years to study mechanisms of retinal processing. However, our knowledge of the synaptic organization of these retinas has not kept pace with the vaster amount of physiological and pharmacological data. It is this gap that the proposal is designed to address. Marc has proposed, on phylogenetic grounds, that the retinas of urodeles can serve as an appropriate model for mammalian retinas. I predict that this project on inhibitory bipolar cells of salamander retinas. I predict that this project on inhibitory bipolar cells of salamander retina will have important implications for studies of inhibitory bipolar cells in rabbit, cat and by extension, primate retinas.

长期目标，如果拟议的研究是了解 脊椎动物内视网膜内的抑制性突触传播。 重点将放在包含抑制性的双极细胞上 Tiger Salamander Retina中的神经递质，GABA（GABA-IR）。 这 要解决的假设是：包含双极细胞的GABA是 持续的抑制性输入到开关的神经节细胞的起源 从而提供解剖基板以进行推动调制 神经节细胞反应。 形态学和电生理技术将用于单个 细胞。 该项目的具体目的是：（1）表征 HRP注射和/或高尔基体染色的双曲线的形态类型。 超级视网膜切片中的单个双极细胞会紧张 在孤立的 视网膜；形态学将与相机Lucida和Eutectic一起研究 神经元跟踪系统； （2）识别神经递质含量（GABA， 标记的双极细胞的不同形态类型的谷氨酸）。 染色细胞的GABA和谷氨酸含量将由 光后免疫荧光双重标记方法 显微镜（EM）水平。 （3）研究突触输入和输出目标 GABA-IR双极的（无小细胞和/或神经节细胞，双极细胞） 电子显微镜（EM）水平的细胞。 结果将被比较 含有谷氨酸的双极细胞； （4）确定突触 GABA-IR双极细胞的连通性以及生理上鉴定的 注射和关闭持续神经节电池的HRP将不在光线 响应将在细胞内记录，神经节细胞将是 随后用HRP染色。 GABA能和谷氨酸能突触 将研究从双极细胞到HRP染色的神经节细胞的输入 使用EM级别的后体免疫金方法。 使用包括salamanders在内的乌罗德勒两栖动物的视网膜已被使用 超过25年来研究视网膜加工的机制。 然而， 我们对这些视网膜突触组织的了解 随着生理和药理数据的变化量。 该提案旨在解决正是这一差距。 马克有 在系统发育的基础上提出的，尿道的视网膜可以使用 作为哺乳动物视网膜的合适模型。 我预测这 关于sal豆视网膜抑制性双极细胞的项目。 我预测 这个关于sal豆视网膜抑制性双极细胞的项目将 对于研究抑制性双极细胞的重要意义 兔子，猫和延伸，灵长类动物。