INHIBITORY SYNAPTIC TRANSMISSION IN THE RETINA

视网膜中的抑制性突触传递

基本信息

批准号：
6384386
负责人：
CHEN-YU YANG
金额：
$ 20.32万
依托单位：
STATE UNIVERSITY NEW YORK STONY BROOK
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-01-01 至 2004-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6384386
关键词：
GABA receptor Urodela amacrine cells electrodes electron microscopy gamma aminobutyrate ganglion cell horseradish peroxidase immunocytochemistry light microscopy morphology neural inhibition neural transmission neuroanatomy receptor binding retinal bipolar neuron synapses voltage /patch clamp

项目摘要

DESCRIPTION (Adapted from the applicant's abstract): The goal of the research is to understand inhibitory synaptic circuitry in the retina that is mediated by a bipolar cell subtype which may be GABAergic. The hypothesis to be addressed is that the four subtypes of GABAergic bipolar cells comprise a single functional unit that synapses upon one type of ganglion cell, the small-simple/ON-OFF type, that is stratified throughout the full thickness of the IPL. Post-embedding electron microscopic immunocytochemistry and whole-cell patch-clamp recording techniques will be used to explore the synaptic circuitry and function of these GABAergic bipolar cells. To this means the study will: (1) characterize the morphology of ganglion cells that demonstrate IPSCs elicited directly by the bipolar cells, followed by labeling with horseradish peroxidase (HRP)/rhodamine for light microscopy and post-embedding immunocytochemical electron microscopy; (2) determine the temporal response properties of the cells by current injection and the polarity of the response type of the filled cells by its dendritic stratification within the IPL; and (3) determine the synaptic organization of the HRP-filled ganglion cells with respect to input from GABA-immunoreactive and non-GABA-immunoreactive bipolar cells, as well as the GABAergic and non-GABAergic amacrine cells.

描述（改编自申请人的摘要）：研究的目的是要了解介导的视网膜中的抑制性突触电路通过双极细胞亚型，可能是GABA能。假设是解决的是，GABA能双极细胞的四个亚型包含A 在一种类型的神经节细胞上突触的单功能单元，小简单/开关类型，在整个厚度的整个厚度 IPL。插后电子显微镜免疫细胞化学和全细胞贴片钳记录技术将用于探索突触电路这些GABA能性双极细胞的功能。这意味着研究将：（1）表征展示IPSC的神经节细胞的形态直接通过双极细胞引起，然后用辣根标记过氧化物酶（HRP）/若丹明，用于光学显微镜和插入后免疫细胞化学电子显微镜；（2）确定时间响应细胞的特性通过电流注入和反应的极性 IPL内的树突分层的填充细胞类型；和（3）确定用HRP填充神经节细胞的突触组织尊重GABA免疫反应性和非GABA免疫反应性双极的输入细胞，以及GABA能和非gaba能无链氨酸细胞。