Spinning Disk Confocal and Single Molecule Localization Microscope

转盘共焦和单分子定位显微镜

• 批准号: 10177496
• 负责人: Jeffrey L Caplan
• 金额: $ 56.17万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-01 至 2022-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10177496
• 关键词: 3-Dimensional; Address; Anabolism; Biogenesis; Biomedical Research; Biotechnology; Cell Wall; Cells; Collagen Fibril; Communities; Confocal Microscopy; Contract Services; Crystalline Lens; Cytoskeleton; Delaware; Ensure; Erythrocytes; Experimental Designs; Fluorescence Microscopy; Funding; Grant; Human Resources; Image; Imaging Techniques; Institutes; Laser Scanning Confocal Microscopy; Light Microscope; Liquid substance; Lung; Measures; Methods; Microscope; Microscopy; Minor; Musculoskeletal Diseases; Organelles; Photobleaching; Phototoxicity; Preparation; Process; Recording of previous events; Research; Research Personnel; Research Project Grants; Resolution; Resources; Sampling; Signal Transduction; Speed; Technology; Tissue Engineering; Uncertainty; United States National Institutes of Health; Universities; bioimaging; bone; data acquisition; design; experience; extracellular vesicles; flexibility; instrument; instrumentation; interest; live cell microscopy; mechanotransduction; meetings; member; multimodality; pathogenic bacteria; programs; single molecule; tissue regeneration; tissue repair

Project Summary / Abstract: 13 NIH-funded investigators are seeking support under the Shared Instrumentation Grant Program to obtain an Andor Dragonfly spinning disk confocal microscope with single molecule localization capability. This multimodal microscope will bring unprecedented speed, resolution, sensitivity and large field of view to the users of the Bio- Imaging Center at the University of Delaware (UD). Spinning disk confocal microscopy is today’s state-of-the-art method for live-cell microscopy due to its reduced photobleaching and phototoxicity, compared to point-scanning confocal microscopy. Furthermore, 3D single molecule localization microscopy is a cutting edge super-resolution imaging technique with that has been attracting widespread interest in biomedical research. Neither of these technologies are currently available to the research community in Delaware, and the Dragonfly is thus set to fill two needed gaps at once. The current application targets key research projects from 10 major users and 8 minor users who have a critical need for the requested microscope. Thanks to its flexibility & multimodality, there is no doubt that this instrument will keep meeting the needs of a wide range of research projects throughout its lifetime. The Dragonfly will impact a broad range of research fields from our major users. This includes Dr. Caplan, who will address fundamental questions on how cellular organelles move and release signals; Drs. Elliott and Wang, who will examine collagen fibrils and bone mechanosensing in respect to musculoskeletal disorders; Dr. Fowler who will study the cytoskeleton in red blood cells and ocular lenses. Other major users include Dr. Gleghorn, who will measure dynamic processes in the developing lung; Dr. Jia, who examine engineered tissue substitutes for tissue repair and regeneration; Drs. Grimes and Neunuebel will study bacterial cell wall biosynthesis and pathogenic bacteria survival and replication in host cells, respectively; Dr. Vlachos will quantify fluid dynamics, and Dr. Tanis will seek to determine the mechanisms underlying the biogenesis of extracellular vesicles. The Dragonfly will be placed in a new state-of-the-art research building, in which imaging suites were specifically designed for high-end light microscopes. It will be managed by the Delaware Biotechnology Institute (DBI)’s Bio- Imaging Center, which is a research hub that serves the imaging needs of over 150 research groups at UD and beyond. DBI and UD have a long established history of providing very strong institutional support, and have committed space, personnel, service contract, and administrative support. A 5-year financial plan has been provided to ensure long-term sustainability. The Bio-Imaging Center has a strong track record for providing excellent and sustained user support and resource management. Four staff members, who have over 75 years of combined experience in fluorescence microscopy, will provide assistance to the Dragonfly’s users in experimental design, sample preparation, data acquisition, and analysis.

项目摘要/摘要： 13 名 NIH 资助的研究人员正在寻求共享仪器资助计划的支持，以获得 Andor Dragonfly 旋转圆盘共聚焦显微镜具有单分子定位功能。 显微镜将为生物显微镜的用户带来前所未有的速度、分辨率、灵敏度和大视野 特拉华大学 (UD) 的成像中心拥有当今最先进的转盘共聚焦显微镜。 与点扫描相比，由于其光漂白和光毒性降低，因此成为活细胞显微镜方法 此外，3D 单分子定位显微镜是一种尖端的超分辨率技术。 这两种成像技术都引起了生物医学研究的广泛兴趣。 特拉华州的研究界目前可以使用这些技术，因此蜻蜓将填补 当前的应用程序针对 10 个主要用户和 8 个次要用户的关键研究项目。 对所需显微镜有迫切需求的用户由于其灵活性和多模态性，因此没有必要。 怀疑该仪器在其整个生命周期中能否持续满足各种研究项目的需求。 Dragonfly 将影响我们主要用户的广泛研究领域，其中包括卡普兰博士。 埃利奥特和王博士将解决细胞器如何移动和释放信号的基本问题； Fowler 博士将检查胶原纤维和骨机械传感与肌肉骨骼疾病的关系； 其他主要用户包括 Gleghorn 博士， 贾博士将测量发育中的肺部的动态过程；他将检查工程组织替代品 Grimes 和 Neunuebel 博士将研究细菌细胞壁的生物合成和再生 Vlachos 博士将量化流体动力学， Tanis 博士将寻求确定细胞外囊泡生物发生的机制。 蜻蜓号将被安置在一座新的最先进的研究大楼内，其中专门配备了成像室 它将由特拉华州生物技术研究所 (DBI) 的 Bio- 管理。 成像中心，这是一个研究中心，为 UD 和 150 多个研究小组的成像需求提供服务 DBI 和 UD 在提供非常强大的机构支持方面有着悠久的历史，并且 承诺的空间、人员、服务合同和行政支持已经制定。 生物成像中心在提供服务方面拥有良好的记录。 优秀且持续的用户支持和资源管理人员，拥有超过 75 年的经验。 荧光显微镜方面的综合经验将为 Dragonfly 用户提供帮助 实验设计、样品制备、数据采集和分析。