New Phosphated Polymers Prevent Microbial Colonization

新型磷酸盐聚合物可防止微生物定殖

• 批准号: 7619626
• 负责人: Andrew R Dentino
• 金额: $ 18.17万
• 依托单位: MARQUETTE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7619626
• 关键词: 2-hydroxyethyl methacrylate; Adherence; Adhesions; Adsorption; Affinity; Animal Model; Biological; Candida albicans; Cell Line; Cells; Characteristics; Charge; Clinical; Clinical Treatment; Communicable Diseases; Defensins; Dental Materials; Dentsply; Denture Bases; Denture Stomatitis; Dentures; Disinfection; Edentulous Mouth; Elasticity; Elderly; Electrostatics; Epithelial Cells; Ethylene Glycols; Exhibits; Fibroblasts; Generations; Gingiva; Goals; Guidelines; Hardness; Human; In Vitro; Infection; Lead; Mechanics; Methacrylates; Oral; Oral health; Organism; Patients; Peptides; Phase; Plant Resins; Polymers; Polymethyl Methacrylate; Population; Porosity; Prevention; Property; Prosthesis; Research; Research Project Grants; Residual state; Resistance; Safety; Saliva; Salivary; Specimen; Stomatitis; Streptococcus; Streptococcus gordonii; Streptococcus mutans; Streptococcus oralis; Streptococcus sanguis; Surface; Surface Properties; Suspension substance; Suspensions; Techniques; Testing; Tissues; Tooth structure; antimicrobial; base; biomaterial compatibility; commercial application; cytotoxic; cytotoxicity; ethylene glycol; histatin 5; histidine-rich proteins; improved; inorganic phosphate; microbial; microbial colonization; microbicide; monomer; oral bacteria; pathogen; polymerization; prevent; research study; tooth surface; water solubility

DESCRIPTION (provided by applicant): The goal of this research project is to develop a new generation of denture-base polymers that could control microbial colonization and prevent denture-induced stomatitis. These new polymers will be synthesized by the incorporation of phosphate groups within poly (methyl methacrylate) [PMMA] to prevent denture infection. Phosphate groups in the new polymers will provide the required anionic surface for the salivary cationic antimicrobials to be selectively adsorbed onto the denture surface. This will result in the formation of a defense pellicle layer on the denture surface. The currently available PMMA, due to the absence of ionic surface and the defense denture pellicle component, does not inhibit microbial colonization This research project involves: 1) Synthesis of phosphated PMMAs containing varied amounts of phosphate groups and non-phosphated PMMA and characterization of polymers; 2) Assessment of the efficacy of this polymer to adsorb and desorb salivary antimicrobials using histatin 5 and a defensin fragment; 3)Determination of the microbicidal activity of antimicrobials adsorbed phosphated polymers using Candida albicans, Streptococcus Sanguis, Streptococcus oralis, Streptococcus gordonii and Streptococcus mutans; 4) Delineation of the physical and mechanical properties of polymers that exhibit high affinity for salivary antimicrobials, and inhibit microbial adhesion to the polymer surface following the ADA/ISO guidelines; 5) Assessment of the in vitro cytotoxicity of polymer specimens against a human oral epithelial cell line, human primary gingival fibroblast cells, and the standard L-929 cell line to ascertain the safety requirements for Dental materials. Relevance: This proposed research will identify new, safe and affordable phosphated PMMA polymers that could serve as denture-base or denture base lining materials for the prevention and treatment of denture infection. These new polymers will exhibit physical, mechanical and biological properties comparable to those of normal oral surfaces, and will have significant impact on the expenses involved in the treatment of denture-induced infection associated with the existing PMMA polymer. The use of these new polymers could improve the oral health of a significant population of prosthesis users. The phosphated PMMA polymers could have great potential for clinical and commercial applications.

描述（由申请人提供）：该研究项目的目的是开发新一代的牙齿碱聚合物，这些聚合物可以控制微生物定植并防止牙齿诱发的口腔炎。这些新聚合物将通过将磷酸基团掺入聚（甲基丙烯酸甲酯）[PMMA]中来合成，以防止义齿感染。新聚合物中的磷酸基团将为唾液阳离子抗菌剂提供所需的阴离子表面，以选择性地吸附到义齿表面上。这将导致义齿表面形成防御层。当前可用的PMMA由于缺乏离子表面和防御义齿膜成分，不会抑制微生物定殖该研究项目涉及：1）磷酸基团的磷酸PMMA的合成，含有多种磷酸盐基团和非磷酸盐基团和非磷酸盐的PMMA和聚合物的特征； 2）评估该聚合物对使用组蛋白5和防御素片段的吸附和解吸唾液抗菌药物的功效； 3）使用白色念珠菌，sanguis链球菌，Oralis链球菌，gordoccoccus gordonii和链球菌链球菌的抗磷酸聚合物吸附的抗菌剂的微生物活性。 4）划定对唾液抗微生物的高亲和力的聚合物的物理和机械性能，并遵循ADA/ISO指南抑制对聚合物表面的微生物粘附； 5）评估聚合物标本的体外细胞毒性针对人口腔上皮细胞系，人类原代牙龈成纤维细胞和标准L-929细胞系的评估，以确定牙科材料的安全要求。相关性：这项拟议的研究将确定可以用作义齿碱或义齿碱衬里材料的新的，安全且负担得起的PMMA聚合物，以预防和治疗牙齿感染。这些新的聚合物将表现出与正常口服表面相当的物理，机械和生物学特性，并将对与现有PMMA聚合物相关的义齿诱导的感染所涉及的费用产生重大影响。这些新聚合物的使用可以改善大量假体使用者的口腔健康。磷酸化的PMMA聚合物可能具有巨大的临床和商业应用潜力。

项目成果

Inhibition of Candida albicans and Mixed Salivary Bacterial Biofilms on Antimicrobial Loaded Phosphated Poly(methyl methacrylate).

• DOI: 10.3390/antibiotics10040427
• 发表时间: 2021-04-13
• 期刊: Antibiotics (Basel, Switzerland)
• 作者: Dentino AR;Lee D;Dentino K;Guentsch A;Tahriri M
• 通讯作者: Tahriri M

Effect of phosphate group addition on the properties of denture base resins.

• DOI: 10.1016/s0022-3913(08)60210-3
• 发表时间: 2008-10
• 期刊: JOURNAL OF PROSTHETIC DENTISTRY
• 影响因子: 4.6
• 作者: Puri, Gaurav;Berzins, David W.;Dhuru, Virendra B.;Raj, Periathamby A.;Rambhia, Sameer K.;Dhir, Gunjan;Dentino, Andrew R.
• 通讯作者: Dentino, Andrew R.

Physical properties of denture base resins potentially resistant to Candida adhesion.

• DOI: 10.1111/j.1532-849x.2007.00219.x
• 发表时间: 2007-11-01
• 期刊: Journal of prosthodontics : official journal of the American College of Prosthodontists
• 作者: Dhir, Gunjan;Berzins, David W;Dentino, Andrew
• 通讯作者: Dentino, Andrew