INTERFERON PROPERTIES, ACTIONS, & PATIENT PRESCREENING

干扰素的特性、作用、

• 批准号: 2089615
• 负责人: Berish Y Rubin
• 金额: $ 18.36万
• 依托单位: FORDHAM UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-30 至 1997-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2089615
• 关键词: DNA directed DNA polymerase; HeLa cells; SDS polyacrylamide gel electrophoresis; aminoacid tRNA ligase; antineoplastics; antisense nucleic acid; antiviral agents; cell type; enzyme induction /repression; enzyme mechanism; gene expression; genetic promoter element; growth inhibitors; human tissue; immunofluorescence technique; immunoperoxidase; immunoprecipitation; interferons; monoclonal antibody; neoplasm /cancer immunotherapy; neoplasm /cancer pharmacology; northern blottings; nucleic acid sequence; protein biosynthesis; radiotracer; tissue /cell culture; transfection; tryptophan; virus replication

Studies will be undertaken to gain further insight into the mechanism of action of the interferons (IFNs). Proposed studies will characterize the antiviral and antiproliferative phenotypes of cells which, as a result of transfection, produce the IFN-induced gp96, 67kD, 56kD, or 42kD proteins. In addition, the cDNAs encoding these proteins will be used to: 1) study the genes encoding these proteins; 2) characterize the promoters responsible for their responsiveness to the IFNs; and 3) determine the chromosomal localization of each gene. Characterization of the IFN-induced 56,000 dalton protein has resulted in the demonstration that this protein is a tryptophanyl tRNA synthetase. Studies proposed will evaluate the relatedness of this protein to the mammalian peptide chain release factor and the role this protein plays in the biological actions of the IFNs. The characterizations of genes and their products that are up-regulated in response to the IFNs are being undertaken to provide insight into the mechanisms by which the IFNs mediate their effects and to enable the development of a methodology by which the responsiveness of tumor tissues to the anticellular effects of the IFNs can be established in vitro prior to their use as therapeutic agents.

将进行研究以进一步了解 干扰素的作用机理（IFNS）。 拟议的研究 将表征抗病毒和抗增殖表型 转染导致的细胞产生的细胞 IFN诱导的GP96、67KD，56KD或42KD蛋白。 另外， 编码这些蛋白质的cDNA将用于：1）研究基因 编码这些蛋白质； 2）表征负责的发起人 因为他们对IFN的响应能力； 3）确定 每个基因的染色体定位。 IFN引起的56,000道尔顿蛋白的表征具有 结果证明了该蛋白是色氨酸 tRNA合成酶。 提出的研究将评估相关性 哺乳动物肽链释放因子和 该蛋白质在IFN的生物学作用中起作用。 基因及其产品的特征 为了对IFN进行响应的上调 提供有关IFN介导的机制的见解 效果并能够开发一种方法论 肿瘤组织对抗细胞作用的反应性 IFN可以在用作治疗之前在体外建立 代理商。