Control of the expression of gonadotropine receptor

促性腺激素受体表达的控制

• 批准号: 09470353
• 负责人: MINEGISHI Takashi
• 金额: $ 1.28万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470353/
• 关键词: Gonadotropin Receptor; Activin; FSH; IGF-I; PRL; mRNA; FSHレセプター; LHレセプター; ルシフェラーゼ活性; ラット顆粒膜細胞; cAMP

FSH is required to maintain FSH and LH receptor at elevated steady state levels after receptor induction in the granulosa cells of diesthystillbesterol (DES)-primed immature rats. To identify the mechanisms underlying the hormone-dependent induction and maintenance of LH receptor (LH-R) in rat granulosa cells, the effect of FSH and some local factors on the LH-R mRNA levels were studied. In order to study the transcriptional regulation of the LH-R, we have isolated a 5' -flanking region of the rat LH-R gene and subcloned nucleotide -1 (relative to the translational initiation codon) to -1379 into a luciferase reporter plasmid. Treatment with 8-Br-cAMP and ESH significantly enhanced activity of 1379 bp of LH-R 5' -flanking region in a dose dependent manner. This data clearly demonstrates that FSH (mediated by CAMP) stimulates the induction of the LH-R in granulosa cells of DES-primed immature rats, at least in part, through increasing the rate of transcription of the LH-R gene. in the presence of FSH, IGF-1 augments FSH receptor mRNA.These results show that IGF-1 is one of the important factors that act in the medium to maintain LH-R levels in granulosa cells.

在二乙基苯甾醇 (DES) 引发的未成熟大鼠的颗粒细胞中进行受体诱导后，需要 FSH 来将 FSH 和 LH 受体维持在升高的稳态水平。为了确定大鼠颗粒细胞中 LH 受体 (LH-R) 激素依赖性诱导和维持的机制，研究了 FSH 和一些局部因素对 LH-R mRNA 水平的影响。为了研究LH-R的转录调控，我们分离了大鼠LH-R基因的5'侧翼区域，并将核苷酸-1（相对于翻译起始密码子）亚克隆到-1379到荧光素酶报告质粒中。用8-Br-cAMP和ESH处理以剂量依赖性方式显着增强LH-R 5'侧翼区1379bp的活性。该数据清楚地表明，FSH（由 CAMP 介导）至少部分通过增加 LH-R 基因的转录速率，刺激 DES 引发的未成熟大鼠的颗粒细胞中 LH-R 的诱导。在存在 FSH 的情况下，IGF-1 会增强 FSH 受体 mRNA。这些结果表明，IGF-1 是在培养基中发挥作用以维持颗粒细胞中 LH-R 水平的重要因素之一。

项目成果

H.Kishi: "Down-Regulation of LH/hCG receptor in rat cultured granulosa cells" FEBS Letters. 402. 198-202 (1997)

H.Kishi：“大鼠培养的颗粒细胞中 LH/hCG 受体的下调”FEBS Letters。

高木 剛: "心疾患合併症の妊娠分娩管理" 日産婦関東連会報. 34(4). 541-549 (1997)

Tsuyoshi Takagi：“心脏病并发症引起的妊娠和分娩的管理”日本妇产科协会关东联盟公告 34(4) 541-549 (1997)。

峯岸 敬: "検査の診断効率とピットフォール : LH, FSH" 中外医学社, 419 (1998)

Takashi Minegishi：“测试的诊断效率和陷阱：LH、FSH”Chugai Igakusha，419 (1998)

T.Minegishi: "Expression of luteinizing hormone/human chorionic gonadotrophin(LH/hCG)receptor mRNA in the human ovary" Molecular Human Reproduction. 3. 101-107 (1997)

T.Minegishi：“人卵巢中黄体生成素/人绒毛膜促性腺激素 (LH/hCG) 受体 mRNA 的表达”《分子人类生殖》。

T.Minegishi: "Expression of follicle-stimulating hormone(FSH)receptor human ovary" European Journal of Clinical Investigation. 27(6). 469-474 (1997)

T.Minegishi：“卵泡刺激素 (FSH) 受体人卵巢的表达”欧洲临床研究杂志。