A&R

A

• 批准号: 10557023
• 负责人: Clarissa A Henry
• 金额: $ 31.97万
• 依托单位: UNIVERSITY OF MAINE ORONO
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-04-05 至 2028-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10557023
• 关键词: Address; Biology; Biomedical Research; Breeding; Candida; Centers of Research Excellence; Circulation; Computers; Cues; Devices; Discipline; Disease; Ecology; Engineering; Faculty; Floor; Funding; Housing; Image Analysis; Influenza; Infrastructure; Laboratories; Larva; Maine; Mechanics; Microbiology; Microscopy; Modeling; Molecular; Muscular Dystrophies; Mycoses; Plumbing; Prevention; Process; Productivity; Pump; Research; Research Personnel; Research Project Grants; Resources; Schools; Science; System; Technology; United States National Institutes of Health; Universities; Water; Work; Zebrafish; base; cell behavior; cell growth regulation; experimental study; extracellular; graduate student; human disease; marine; new growth; rapid growth; response; teaching laboratory; undergraduate student

Alteration and Renovation Abstract UMaine’s Zebrafish Facility is critical to the majority of the Research Project Leaders (RPLs) on this COBRE. Overall, 12 UMaine faculty, their technicians, undergraduate, and graduate students use the Zebrafish Facility for their research on an annual basis. These faculty represent a number of different units at UMaine including the Department of Molecular and Biomedical Sciences, the School of Biology and Ecology, and the School of Marine Sciences. Zebrafish have been used by UMaine faculty studying many different disease processes, including influenza, muscular dystrophy, and Candida fungal infections. The Zebrafish Facility is also relevant to UMaine faculty in disciplines such as electrical and computer engineering, who are developing devices and technologies to address the prevention and treatment of human disease processes. UMaine’s current zebrafish facility, located in the same building as the UMaine RPLs and the proposed Microscopy and Image Analysis Core, typically operates at 98-100% capacity. Although an excellent facility that is 93% base-funded by the University of Maine; the limited capacity hinders all investigators who use the zebrafish model. This facility is an essential resource for 3 COBRE RPLs to maintain and develop zebrafish lines used for their proposed research projects. As part of our COBRE we are proposing to expand UMaine’s Zebrafish to accomplish the following: increase nursery space to facilitate rapid growth of new lines, increase spawning space to increase experimental pace, and nearly double the capacity of our current system. We will do this by completing two specific aims. Specific Aim 1: Convert a Teaching Laboratory into an Additional Zebrafish Facility. This renovation project will convert a 1,242 ft2 teaching laboratory in Hitchner Hall, that is across the hall from the current facility, into an additional Zebrafish Laboratory. The addition of this lab to the overall zebrafish facility will more than double the available space for zebrafish housing. Renovations will include demolition of the current space, sloping of the floor, installation of a trench drain, HVAC, electrical work, and installation of a heat pump. Specific Aim 2: Purchase a centralized water circulation system, tank racks, and tanks. Within the scope of COBRE funding, as well as with a commitment of $154,000 from the Office of the Vice President for Research at UMaine, we will install the centralized infrastructure for the final number of tank racks, the immediate purchase and installation of racks and tanks that will immediately increase our capacity by 91%. Purchase of crossing shelves will increase daily breeding capacity from 25 to 175 tanks per day. This increased capacity is necessary for the COBRE research project leaders (RPLs) that use the zebrafish model and will be transformative for all of the zebrafish researchers at UMaine.

改变和翻新摘要 Umaine的斑马鱼设施对大多数研究项目负责人（RPL）至关重要。 总体而言，12名Umaine教职员工，他们的技术人员，本科生和研究生使用斑马鱼设施 每年的研究。这些教师代表Umaine的许多不同单位，包括 分子与生物医学科学系，生物学与生态学院以及 海洋科学。乌梅恩（Umaine）研究了许多不同的疾病过程， 包括影响，肌肉营养不良和念珠菌真菌感染。斑马鱼设施也很重要。 向Umaine教职员工，例如电气和计算机工程，他们正在开发设备和 解决人类疾病过程的预防和治疗的技术。 Umaine目前的斑马鱼设施，位于与Umaine RPL的同一建筑物中 显微镜和图像分析核心通常以98-100％的容量运行。虽然是一个出色的设施 这是缅因大学获得的93％的基础资助；有限的容量阻碍了所有使用的调查员 斑马鱼模型。该设施是3个cobre Rpls维护和开发斑马鱼的重要资源 用于其建议的研究项目的线路。作为我们的毛病的一部分，我们建议扩大Umaine的 斑马鱼要完成以下操作：增加苗圃空间以促进新线路的快速增长，增加 产卵空间以增加实验空间，几乎是我们当前系统的容量的两倍。我们将 通过完成两个具体目标来做到这一点。特定目标1：将教学实验室转换为其他 斑马鱼设施。这个翻新项目将在希奇纳音乐厅转换1,242 FT2教学实验室，即 从当前工厂穿过整个大厅，进入了另一个斑马鱼实验室。该实验实验室的添加到 斑马鱼的总体设施将是斑马鱼住房的可用空间的两倍以上。翻新将 包括拆除当前空间，地板倾斜，沟槽排水管的安装，HVAC，电动 工作和安装热泵。特定目标2：购买集中式水流通系统， 坦克架和坦克。在毛茸茸的资金范围内，以及从$ 154,000的承诺 Umaine研究副总裁办公室，我们将安装集中式基础设施 储罐架数量，立即购买和安装架子和储罐 将我们的容量增加91％。购买货架的购买将使每日繁殖能力从25增加到 每天175个坦克。这种增加的能力是毛茸茸的研究项目负责人（RPLS）所必需的。 使用斑马鱼模型，并将对Umaine的所有斑马鱼研究人员进行变革。