Functions of gustatory afferent neuron types.

味觉传入神经元类型的功能。

基本信息

批准号：
10541809
负责人：
Nirupa Chaudhari
金额：
$ 60.8万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-07-10 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10541809
关键词：
Afferent Neurons Auditory Brain Brain Stem Categories Cell Communication Cell Nucleus Cells Cephalic Chromium Code Data Detection Diet Eating Electrophysiology (science)Entropy Feeding behaviors Food Selections Funding Ganglia Gene Expression Profile Genetic Genomics Goals Image Insulin Label Laboratories Lasers Measures Mediating Methods Molecular Molecular Profiling Mouse Strains Mus Neural Pathways Neurons Noise Pathway interactions Phase Physiological Process Reflex action Reticular Formation Retina Retinal Ganglion Cells Signal Transduction Stimulus Structure of geniculate ganglion System Taste Bud Cell Taste Buds Taste Perception Testing Visual biomarker validation cell type combinatorial designer receptors exclusively activated by designer drugs food consumption glucose tolerance in vivo molecular marker nutrition prototype response single-cell RNA sequencing somatosensory taste stimuli transcriptome transmission process

项目摘要

Project Summary Single-cell RNA sequencing (scRNAseq) has made unprecedented strides in discovering previously unrecognized diversity of neuronal cell types and their functions. Using scRNAseq, we showed that the mouse geniculate ganglion contains 3 molecularly distinct types of gustatory neurons that innervate taste buds, T1, T2, and T3, each with unique patterns of gene expression. In a concerted effort from two well-established laboratories, we now propose a multi-PI project to test hypotheses regarding distinct functions for each of the major types of neurons and their subtypes. Our ultimate goal is to produce an integrated molecular and functional categorization of gustatory neurons similar to what has been so powerfully effective in the auditory, visual, and somatosensory systems. We propose using a newly-optimized method for in vivo confocal Ca2+ imaging, neuron-selective fluorescent markers, and chemogenetic silencing to reveal the functions of T1 and T3 geniculate ganglion neurons. Specifically, using GCaMP-based Ca2+ imaging, we will test the hypothesis that within the cluster of T1 neurons there are subclasses that respond to distinct taste qualities whereas neurons within the T3 cluster respond to multiple, convergent tastes. We will further test the hypothesis that T1 and T3 neuronal subtypes contribute to separate central pathways serving a number of taste-dependent functions downstream of initial detection in the taste periphery. Completing the above aims will move the field of taste into a new era of molecular-functional integration. Our findings will assist electrophysiology and circuit tracing studies in taste, will shed light on the controversy over labeled lines versus combinatorial taste coding, and will bring new information on gustatory neural pathways that are so important to nutrition and ingestive behavior.